In the original publication [1], there was a mistake in Figure 7. Figure 7B,C (Untreated MCF7 Cells) and Figure 7D,E (Blank nano particle @2h) have, in fact, very low uptake and have almost the same data. However, when changing to drug-loaded nanoparticles and transferrin-conjugated drug-loaded nanoparticles, the uptake increases. When building the combined image, the panels D and E were misplaced. The correct Figure 7 appears below. The authors state that the scientific conclusions are unaffected. This correction was approved by the Academic Editor. The original publication has also been updated.
Figure 7.
Cellular uptake study using flow cytometry: (A) gating, (B,C) fluorescence of untreated MCF-7 cells, (D,E) fluorescence of blank nanoparticles at 2 h, (F,G) fluorescence of blank nanoparticles at 24 h, (H,I) fluorescence of unconjugated nanoparticles at 2 h, (J,K) fluorescence of unconjugated nanoparticles at 24 h, (L,M) fluorescence of DCT-loaded Tf-conjugated PLGA NPs at 2 h, (N,O) fluorescence of Tf-conjugated PLGA NP at 24 h. The x- and y-axes correspond to forward scatter (FSC) (which measures size) and side scatter (SSC) (which measures internal complexity), respectively. The FL1-area stands for total cell fluorescence.
Footnotes
Disclaimer/Publisher’s Note: The statements, opinions and data contained in all publications are solely those of the individual author(s) and contributor(s) and not of MDPI and/or the editor(s). MDPI and/or the editor(s) disclaim responsibility for any injury to people or property resulting from any ideas, methods, instructions or products referred to in the content.
Reference
- 1.Jose S., Cinu T.A., Sebastian R., Shoja M.H., Aleykutty N.A., Durazzo A., Lucarini M., Santini A., Souto E.B. Transferrin-Conjugated Docetaxel–PLGA Nanoparticles for Tumor Targeting: Influence on MCF-7 Cell Cycle. Polymers. 2019;11:1905. doi: 10.3390/polym11111905. [DOI] [PMC free article] [PubMed] [Google Scholar]