Figure 8.

(a) Interleukin (IL)-1α and IL-6 protein expression and (b) IL-8 protein expression in atopic dermatitis full-thickness skin models after days 7 to 9 and days 9 to 12 of cultivation to air surface (airlift). Atopic dermatitis skin models were stimulated with T_H2 cytokines (50 ng/mL IL-4, 50 ng/mL IL-13 and 25 ng/mL IL-31) at days 0, 2, 5, 7 and 9 (m2, m3). Atopic dermatitis skin model m3 was additionally stimulated with 10 µM histamine at day 9. Skin models m2 and m3 were pre-incubated with either 5 µM α-13’-COOH, 25 µM α-13’-COOH, 1 µM dexamethasone (Dex; positive control), or 0.5% DMSO (vehicle control) at days 7 and 9, or were left untreated as disease control (untreated). Cytokine levels are given as fold change to untreated disease control at the respective time points, after day 9 (incubation period days 7 to 9) and day 12 (incubation period days 9 to 12) of incubation. (a) All data in the heatmap are presented as means. (b) All data are presented as means ± SEM. Two independent experiments were performed as replicates. Asterisks [*] indicate significant deviations from the untreated disease control at the respective time point. (* p < 0.05, ** p < 0.01 and *** p < 0.001).