Fig. 7.

Drug evaluation and identification using the H/R model on ICOs. ICOs (n = 3) were stimulated with 72-h hypoxia (HY), followed by 6-h reoxygenation (RE), in the presence or absence of emricasan (20 μM) or A1AT (20 mg/mL). (a and b) Representative bright-field images of the treated ICOs are shown. (c–f) Stimulated ICOs (n = 3) were fixed and stained for active caspase 3 and pMLKL, along with DAPI/F-actin staining. Representative fluorescent images are shown. Enlarged images from the boxed area are shown in the right panels. (g and h) Quantification of positive cells in fluorescent images was performed using Image J (at least 8 HPF in each ICOs line and condition). Data are mean ± SD, ∗∗p < 0.01, ∗∗∗p < 0.001, Kruskal–Wallis test followed by Dunn's post hoc test (g and h).