REAGENT	SOURCE	IDENTIFIER
Antibodies
goat anti-Pax2 (1:200)	R and D Systems	Cat#AF3364; RRID:AB_10889828
goat anti-CGRP (1:500)	Abcam	Cat#ab36001; RRID:AB_725807
goat anti-tdTomato (1:1000)	SICGEN	Cat# AB8181-200; RRID:AB_2722750
goat anti-TrkA (1:400)	R & D Systems	Cat#AF1056; RRID:AB_2283049
goat anti-WGA	Vector Laboratories	Cat#AS-2024; RRID:AB_2315608
guinea pig anti-Lmx1b (1:10.000)	Müller et al., 2002	N/A
mouse anti-NeuN (1:500)	Millipore	Cat#MAB377; RRID:AB_2298772
rabbit anti-cfos (H125) (1:500)	Santa Cruz Biotechnology	Cat#SC7202; RRID:AB_2106765
rabbit anti-Cmaf (1:10.000)	Wende et al., 2012	N/A
rabbit anti-Gbx1 (1:5000)	John et al., 2005	N/A
rabbit anti-GFP (1:1000)	Molecular Probes	Cat#A6455; RRID:AB_221570
Rabbit anti-NeuN (1:3000)	Abcam	Cat#ab104225; RRID:AB_10711153
rabbit anti-NF200 (1:1000)	Sigma	Cat#N4142; RRID:AB_477272
rabbit anti-P2X3 (1:1000)	Abcam	Cat#ab10269; RRID:AB_297006
rabbit anti-PKCγ (1:1000)	Santa Cruz Biotechnology	Cat#sc-211; RRID:AB_632234
rabbit anti-PV (1:2000)	Swant	Cat#PV25; RRID:AB_10000344
rabbit anti-WGA (1:2000)	Sigma-Aldrich	Cat#T4144; RRID:AB_261669
sheep anti-PlexinC1 (1:400)	R & D Systems	Cat#AF5375; RRID:AB_2284038
sheep anti-TH (1:1000)	Millipore	Cat# AB1542; RRID:AB_90755
Alexa Fluor 488-donkey anti-goat (1:800)	Jackson ImmunoResearch	Cat#795-546-147; RRID:AB_2340430
Alexa Fluor 488-donkey anti-rabbit (1:800)	Jackson ImmunoResearch	Cat#711-546-152; RRID:AB_2340619
Alexa Fluor 647-donkey anti-goat (1:800)	Jackson ImmunoResearch	Cat#705-605-147; RRID:AB_2340437
Alexa Fluor 647-donkey anti-guinea pig (1:800)	Jackson ImmunoResearch	Cat#706-496-148; RRID:AB_2340477
Alexa Fluor 647-donkey anti-rabbit (1:800)	Jackson ImmunoResearch	Cat#711-607-003; RRID:AB_2340626
DyLight™ 647-donkey anti-sheep (1:800)	Jackson ImmunoResearch	Cat#713-606-147; RRID:AB_2340752
Fluorescent conjugates
Alexa Fluor 647-Isolectin GS-IB4 (1:500)	Thermo Fisher Scientific	Cat#I32450; RRID:SCR_014365
Bacterial and virus strains
AAV1.EF1a.flex.DTA (3.3 × 1012 IU/ml)	Foster et al., 2015	N/A
AAV1.hSyn.flex.hM3Dq-mCherry (6.5 × 1012 IU/ml)	Foster et al., 2015	N/A
AAV2.Ef1a.flex.WGA (5.3 × 1012 IU/ml)	UNC Vector Core	N/A
AAV1.Ef1a.flex.eGFP (4.7 × 1012 IU/ml)	VVF Zurich	v217
Chemicals, peptides, and recombinant proteins
clozapine-N-oxide (CNO)	Enzo Life Sciences	BBL-NS105-0025
DTX	Millipore	Cat. No. 322326
icilin	Sigma	i9532-10mg
(-)-menthol	Merck	Cat. No. 63660-100G
Critical commercial assays
Mm-Kcnip2-C3	Advanced Cell Diagnostics	Cat No. 536361-C3
Mm-Slc32a1-C2	Advanced Cell Diagnostics	Cat No. 319191-C2
tdTomato	Advanced Cell Diagnostics	Cat No. 317041
RNAscope Fluorescent Multiplex Reagent Kit	Advanced Cell Diagnostics	Cat No. 320850
Deposited data
Raw data	This paper	https://doi.org/10.5281/zenodo.7143887
Experimental models: Organisms/strains
Mouse: GlyT2::Dre	This paper	N/A
Mouse: Hoxb8::Dre	This paper	N/A
Mouse: Kcnip2roxCre	This paper	N/A
Mouse: ROSA26lsl-iDTR	The Jackson Laboratory	RRID:IMSR_JAX:007900
Mouse: ROSA26lsl-tdTom	The Jackson Laboratory	RRID:IMSR_JAX:007914
Mouse: ROSA26rsr-tdTom	This paper	Derived from RRID:IMSR_JAX:021876
Software and algorithms
GraphPad Prism version 5	GraphPad Software	https://www.graphpad.com/scientific-software/prism/
Fiji ImagJ	Schindelin et al., 2012	https://imagej.net/software/fiji/
CineLAB	Plexon Inc.	https://plexon.com/cinelab-video-tracking-system/