Correction to: Editing of Mitochondrial Transcripts nad3 and cox2 by Dek10 Is Essential for Mitochondrial Function and Maize Plant Development

This is a correction to: Weiwei Qi, Zhongrui Tian, Lei Lu, Xiuzu Chen, Xinze Chen, Wei Zhang, Rentao Song, Editing of Mitochondrial Transcripts nad3 and cox2 by Dek10 Is Essential for Mitochondrial Function and Maize Plant Development, Genetics, Volume 205, Issue 4, 1 April 2017, Pages 1489–1501, https://doi.org/10.1534/genetics.116.199331

In the originally published version of this manuscript, there was a lack of clarity regarding the immunoblot methods and how they related to Figure 3F and Figure 5C in which the same internal control panel (anti-Tubulin) was used.

Accordingly, the Materials and Methods section “Immunoblot Analysis” should contain this additional information:

First, the extracts from WT and dek10 kernels were separated on a protein gel and analyzed by immunoblot with anti-Tubulin in order to normalize loading. Following this, the same size aliquots from the same extracts used in the Tubulin control were run on independent gels and blotted. Each blot was probed with a single antibody: anti-Dek10, anti-Nad3, anti-Cox2 or anti-Cyt C. Because anti-Tubulin had been used to normalize the same batch of WT and dek10 protein extracts, and the same size aliquots of WT and dek10 extracts as in the initial anti-Tubulin gel were loaded on the subsequent gels/blots, the anti-Tubulin loading control was relevant to both Fig 3F and Fig 5C; it was therefore included in both figures.

The legend for Figure 5 should read:

Dek10 is required for nad3-61, nad3-62, and cox2-550 editing in maize mitochondria. (A) Analysis of RNA editing at the nad3-61, nad3-62, and cox2-550 sites in the transcripts from developing kernels of the WT, dek10-ref, and dek0-Cas9 mutants at 18 DAP. The arrow marks the editing site. (B) Alignment of the Nad3 sequences around amino acid 21 and alignment of the Cox2 sequences around amino acid 184. The protein sequences are derived from Z. mays, Sorghum bicolor, O. sativa, Lolium perenne, T. aestivum, Beta vulgaris, Nicotiana sylvestris, B. napus, M. truncatula, and Arabidopsis thaliana. Numbers indicate amino acid positions in the protein. The red box indicates the position affected by the nad3-61, nad3-62, and cox2-550 editing. (C) Western blot analysis with antibodies against Nad3, Cox2, and Cyt-c. Anti-Tub was used as sample loading control. (D) BN-PAGE of mitochondrial complexes. The positions of super complex I+III², complex I, complex III, and complex IV are indicated. (E) In-gel NADH dehydrogenase activity test analysis of complex I activity. The positions of super complex I+III² and complex I are indicated. The activity of the dehydrolipoamide dehydrogenase was used as a sample loading control. BN-PAGE, blue native polyacrylamide gel electrophoresis; Cox, Cyt-c oxidase; Cyt-c, cytochrome c; DAP, days after pollination; WT, wild type. The anti-Tubulin panel in 5C is the same as that shown in 3F for the reasons explained in the correction regarding Materials and Methods.

These details have been corrected only in this correction notice to preserve the published version of record.