Extended Data Fig. 5. Conformational changes in Insertion domain upon target RNA binding.

a, Cas12a2 autoinhibited binary complex (left) and active ternary complex (right), colored as in Fig. 1a. Insertion domain is dark cyan. b, conformational changes of the C-terminal half of insertion domain from binary (grey) to ternary (dark cyan) complexes. N-terminal half remains static, and interacts with the crRNA 5′ handle.