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. 2023 Jan 4;613(7944):534–542. doi: 10.1038/s41586-022-05562-8

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© The Author(s) 2023, corrected publication 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 8 — a–d, Examples of VPN cell types with retinotopic mapping of axon terminals reflecting the AP axis of the lobula dendritic map. Assessed via EM reconstructions and light microscopy (individual cells are labeled using Multicolor Flp-Out). LC4 neurons (a, same pair as in Fig. 5a) with dendrites innervating anterior and posterior lobula have axon terminals in distinct regions of the glomerulus. LC9 (b), LC22 (c), LPLC4 and LPLC1 (d) show similar axonal topography. Left panels, hemibrain connectome reconstructions of 15 anterior (red), 15 posterior (blue), and the remaining cells (grey). Corresponding reconstruction of axons in the VPN glomeruli shows visual separation of anterior and posterior terminals. Right panels: one anterior (red) and one posterior (green) cell labeled using Multicolor Flp-Out and assessed via light microscopy. (n = 4 pairs of A-P individual clones from different brains with reproducible axon terminal topography for LPLC4 and LPLC1, n = 5 for LC9, n = 6 for LC22). e, Traces of DV axonal retinotopy in LC16 – a single example found across 20 VPN cell types. f, Representative examples of VPNs without topographic mapping of axon terminals in optic glomeruli (EM reconstructions), despite elaborating synaptic gradients reflecting visual space map (see Fig. 4h and Extended Data Fig. 7). g–j, Differential axon terminal morphology and glomerular targeting between individual LC4 (g, h) and LC22 neurons (i, j) innervating anterior and posterior regions of the lobula (N = 9 pairs of A-P individual clones from different brains with reproducible axon terminal topography for LC4, n =64 for LC22, correspond to examples from Fig. 5). Characteristic branching patterns are consistent between light microscopy and EM-based connectome reconstruction. A, anterior; P, posterior; D, dorsal; V, ventral; L, lateral; M, medial.