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. 2023 Jan 4;613(7944):534–542. doi: 10.1038/s41586-022-05562-8

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© The Author(s) 2023, corrected publication 2023

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Extended Data Fig. 10 — a, Top: EM-based connectome reconstructions of LC4 neurons (green) and three DNs. Bottom: confocal projections of colocalized LC4 and three DNs, LC4 glomerulus is indicated with a dashed yellow rectangle (n = 12 brains for each LC4-DN, corresponding to Fig. 5c,d). Note that DNp11 has an additional dendritic branch in the lobula. b, Imaris reconstructions of confocal image stacks: LC4 glomerulus (axons) and dendritic segments of three DNs (both reconstructed as filaments) as indicated. c, Assessment of spatial distribution of DN dendrites within the volume of the LC4 glomerulus (outlined with a green dashed line). Topographic separation of the LC4 axon terminals occurs along the sagittal diameter of the glomerulus. Normalized value of the sagittal diameter was used to assess the relative placement of the postsynaptic dendrites (see Methods). Dotted straight lines indicate the positions of DN dendritic centroids along the sagittal diameter of the glomerulus. Position of the LC4 glomerulus centroid slightly deviates from 0.5 value due to the naturally curved shape of the glomerulus. d, Strategy for sparse labeling of LC4 neurons and their presynaptic sites. Labeling of cell membranes (myr::GFP) and presynaptic sites (Brp-smGdP-V5) is dependent upon heat-shock induced expression of FLP (See Methods). e, Confocal projection of a single LC4 neuron with presynaptic sites labeled and colocalized with dendrite of DNp02 (n = 18 individual LC4 neurons from different brains, corresponding to Fig. 6e–h. f, Confocal projection of a single LPLC2 neuron with labeled presynaptic sites colocalized with GF dendrite (n = 10 individual LPLC2 neurons from different brains, corresponding to Fig. 6k,l). Regions corresponding to LC4 and LPLC2 glomeruli are indicated with dashed yellow rectangles.