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. 2023 Jan 6;13(1):120. doi: 10.3390/biom13010120

Table 3.

Primers used for RT-qPCR: sequences and binding temperatures (Temp).

GENE SEQUENCE Fwd (5′→3′) SEQUENCE Rev (5′→3′) Temp
TBP TGCACAGGAGCCAAGAGTGAA CACATCACAGCTCCCCACCA 60 °C
TFRC GAACTACACCGACCCTCGTG GTGCTGTCCAGTTTCTCCGA 60 °C
ACSL-4 GCTGGGACAGTTACTGAAGGT AGAGATACATACTCTCCTGCTTGT 58 °C
ALOX-5 TGGCGCGGTGGATTCATAC AGGGGTCTGTTTTGTTGGCA 60 °C
GPX4 ATTGGTCGGCTGGACGAG CCGAACTGGTTACACGGGAA 59 °C