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. 2022 Dec 22;13(1):23. doi: 10.3390/biom13010023

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Nucleofection of HUVECs with a titration of EGFP mRNA. (a) Visualization of the EGFP positive cells by fluorescent- and bright field microscopy. (b) The profiles of EGFP-positive HUVECs at each EGFP mRNA concentration. Values are presented as normalized counts of the fluorescent intensity. (c) Fraction of EGFP-positive HUVECs at each EGFP mRNA concentration. Values are presented as the mean percentage of EGFP-positive cells $\pm$ SD. Neighboring EGFP mRNA concentrations are compared by one-way ANOVA with Tukey multiple comparisons test, ** p ≤ 0.01, **** p ≤ 0.0001. (d) Median fluorescence intensity of EGFP-positive cells at each EGFP mRNA concentration. Values are presented as the mean $\pm$ SD. Neighboring EGFP mRNA concentrations are compared by one-way ANOVA with Tukey multiple comparisons test, * p ≤ 0.05, ** p ≤ 0.01, **** p ≤ 0.0001. (a–d): Experiments on HUVECs from one biological donor at p. 5 (n = 3).