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. 2022 Dec 23;13(1):26. doi: 10.3390/metabo13010026

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Metabolic pathway analysis performed for metabolites significant in different comparisons: 0 vs. 6 (panel A), 0 vs. 9 (panel B), 0 vs. 12 (panel C), and 0 vs. 18 (panel D). Pathway analysis includes data from comparisons with EDTA and non-EDTA together. Significant pathways marked with the numbers are panel A—1. Glycerophospholipid metabolism, 2. Glycerolipid metabolism, 3. Ether lipid metabolism, and 4. Phosphatidylinositol signaling system; panel B—1. Ether lipid metabolism, 2. Pyruvate metabolism, 3. Glycolysis/Gluconeogenesis, 4. Glutathione metabolism, 5. Glycerophospholipid metabolism, and 6. Purine metabolism; panel C—1. Glycerophospholipid metabolism, 2. Linoleic acid metabolism, 3. alpha-Linolenic acid metabolism, 4. Glycerolipid metabolism, 5. Ether lipid metabolism, 6. Phosphatidylinositol signaling system, 7. Glutathione metabolism, 8. Biosynthesis of unsaturated fatty acids, and 9. Purine metabolism; panel D—1. Glycerophospholipid metabolism, 2. Linoleic acid metabolism, 3. alpha-Linolenic acid metabolism, 4. Glycerolipid metabolism, 5. Histidine metabolism, 6. Pantothenate and CoA biosynthesis, and 7. Ether lipid metabolism.