Table 2.

Different treatments on the CFS of L. animalis SWLA-1 and residual antimicrobial activity.

Treatment	Condition		Final pH	Residual Antimicrobial Activity (%)
Concentration				S. gallinarum	S. enteritidis	E. coli	S. aureus
CFS (1×)	None		5.1	100 $\pm$ $0.0$	100 $\pm$ $0.0$	100 $\pm$ $0.0$	100 $\pm$ $0.0$
Concentrated (10×)	Lyophilized, 24 h		5.1	400 $\pm$ $0.0$	400 $\pm$ $0.0$	400 $\pm$ $0.0$	400 $\pm$ $0.0$
Temperature
40 °C	Heated, 2 h		5.1	100 $\pm$ $0.0$	100 $\pm$ $0.0$	100 $\pm$ $0.0$	100 $\pm$ $0.0$
60 °C	Heated, 2 h		5.1	100 $\pm$ $0.0$	100 $\pm$ $0.0$	100 $\pm$ $0.0$	100 $\pm$ $0.0$
80 °C	Heated, 2 h		5.1	100 $\pm$ $0.0$	100 $\pm$ 16.7	100 $\pm$ $0.0$	100 $\pm$ $0.0$
100 °C	Heated, 30 min		5.1	83.3 $\pm$ 16.7	83.3 $\pm$ 16.7	100 $\pm$ $0.0$	100 $\pm$ $0.0$
pH
2.0	Exposure to pH 2 at 37 °C, 2 h		6.8	50 $\pm$ $0.0$	50 $\pm$ $0.0$	50 $\pm$ $0.0$	50 $\pm$ $0.0$
4.0	Exposure to pH 4 at 37 °C, 2 h		6.8	50 $\pm$ $0.0$	50 $\pm$ $0.0$	50 $\pm$ $0.0$	50 $\pm$ $0.0$
6.0	Exposure to pH 6 at 37 °C, 2 h		6.8	50 $\pm$ $0.0$	25 $\pm$ $0.0$	50 $\pm$ $0.0$	50 $\pm$ $0.0$
8.0	Exposure to pH 8 at 37 °C, 2 h		6.8	50 $\pm$ $0.0$	25 $\pm$ $0.0$	50 $\pm$ $0.0$	50 $\pm$ $0.0$
Enzyme
Proteinase K	2 mg/mL at 37 °C, 2 h		5.1	100 $\pm$ $0.0$	66.7 $\pm$ 16.7	100 $\pm$ $0.0$	100 $\pm$ $0.0$
Trypsin	1 mg/mL at 37 °C, 2 h		5.1	83.3 $\pm$ 16.7	66.7 $\pm$ 16.7	83.3 $\pm$ 16.7	83.3 $\pm$ 16.7

Each value is presented as the mean $\pm$ standard deviation.