Figure 2.
Validation of wristwatch PCR by walking the selected genes
Genome-walking for gadA of L. brevis CD0817 (A) and hyg of rice (B). I, II, and III represent three sets of WW-PCRs in a walking, individually participated by the three WWP permutations as indicated in Table 2. Lanes P, S, and T represent primary, secondary, and tertiary PCRs, respectively. Bands GS1-GS14 and GT1-GT13 indicate secondary and tertiary PCR products for gadA, respectively; and bands HS1-HS4 and HT1-HT4 indicate secondary and tertiary PCR products for hyg, respectively. M: DL 5000 DNA Marker (5000, 3000, 2000, 1500, 1000, 750, 500, 250, and 100 bp). This figure is reprinted from our previous publication.1 Copyright of © 2022 Wang, Jia, Li, Liu, Sun, Pei, Wei, Lin and Li.