| Antibody |
Mouse monoclonal anti-α-synuclein |
BD Biosciences |
Cat# 610787 |
(1:500) |
| Antibody |
Rabbit polyclonal anti-α-synuclein |
Proteintech |
Cat# 10842-1-AP |
(1:500) |
| Antibody |
Rabbit polyclonal anti-DNAJC5 |
RayBiotech |
Cat# 144-10489-200 |
(1:1,000) |
| Antibody |
Mouse monoclonal anti-alpha tubulin |
Abcam |
Cat# ab7291 |
(1:2,000) |
| Antibody |
Mouse monoclonal anti-Alix |
Santa Cruz Biotechnology |
Cat# Sc-53540 |
(1:1,000) |
| Antibody |
Rabbit monoclonal anti-CD9 |
Cell Signaling Technology |
Cat# 13174S |
(1:1,000) |
| Antibody |
Mouse monoclonal anti-PDI |
Enzo Life Sciences |
Cat# ADI-SPA-891-D |
(1:1,000) |
| Antibody |
Mouse monoclonal anti-CD63 |
Thermo Fisher Scientific |
Cat# BDB556019 |
(1:1,000) |
| Antibody |
Mouse monoclonal anti-Flotillin-2 |
BD Biosciences |
Cat# 610383 |
(1:1,000) |
| Antibody |
Mouse monoclonal anti-Transferrin Receptor |
Thermo Fisher Scientific |
Cat# 13-6800 |
(1:1,000) |
| Antibody |
Mouse monoclonal anti-GM130 |
BD Biosciences |
Cat# 610823 |
(1:1,000) |
| Antibody |
Rabbit monoclonal anti-Tom20 |
Cell Signaling Technology |
Cat# 42406S |
(1:1,000) |
| Antibody |
Rabbit polyclonal anti-GFP |
Fisher Scientific |
Cat# NC9589665 |
(1:1,000) |
| Antibody |
Rabbit polyclonal anti-LC3B |
Novus Biologicals |
Cat# NB100-2220 |
(1:1,000) |
| Antibody |
Rabbit monoclonal anti-Citrate Synthase |
Cell Signaling Technology |
Cat# 14309S |
(1:1,000) |
| Antibody |
Rabbit polyclonal anti-Dopamine transporter |
Bioss Antibodies |
Cat# BS-1714R |
(1:1,000) |
| Antibody |
Rabbit monoclonal anti-beta III Tubulin |
Abcam |
Cat# ab215037 |
(1:1,000) |
| Antibody |
Rabbit polyclonal anti-Tyrosine hydroxylase |
Millipore |
Cat# AB152 |
(1:1,000) |
| Antibody |
Chicken polyclonal Microtubule-associated protein 2 |
Abcam |
Cat# ab92434 |
(1:1,000) |
| Antibody |
Mouse monoclonal anti-FLAG |
Sigma-Aldrich |
Cat# F9291 |
(1:1,000) |
| Strain, strain background (Escherichia coli) |
XL1-Blue competent cells |
MacroLab Berkeley |
N/A |
|
| Strain, strain background (E. coli) |
Rossetta (DE3) pLysS competent cells |
MacroLab Berkeley |
N/A |
|
| Chemical compound, drug |
Anti-FLAG M2 Affinity Gel |
Sigma-Aldrich |
Cat# A2220-5ML |
|
| Chemical compound, drug |
Dimethyl sulfoxide (DMSO) |
Thermo Fisher Scientific |
Cat# BP231-100 |
|
| Chemical compound, drug |
Quercetin |
Sigma-Aldrich |
Cat# Q4951-10G |
|
| Chemical compound, drug |
2-Bromopalmitic acid |
Millipore Sigma |
Cat# 21604-1G |
|
| Chemical compound, drug |
Balfilomycin A1 |
Cayman Chemical |
Cat# 11038 |
|
| Chemical compound, drug |
Retinoic acid |
Sigma-Aldrich |
Cat# R2625-100MG |
|
| Chemical compound, drug |
HaloTag Oregon Green Ligand |
Promega |
Cat# G2802 |
|
| Chemical compound, drug |
HaloTag TMR Ligand |
Promega |
Cat# G8251 |
|
| Chemical compound, drug |
Prolong Gold with DAPI |
Thermo Fisher Scientific |
Cat# P36931
|
|
| Chemical compound, drug |
Proteinase K |
Sigma-Aldrich |
Cat# P2308 |
|
| Peptide, recombinant protein |
α-syn tandem repeats protein |
This paper |
N/A |
|
| Commercial assay or kit |
Nano-Glo Luciferase Assay System |
Promega |
Cat# N1150 |
|
| Commercial assay or kit |
LEGEND MAX Human α-synuclein (Colorimetric) ELISA Kit |
BioLegend |
Cat# 448607 |
|
| Commercial assay or kit |
Mouse α-synulcein ELISA Kit |
Abcam |
Cat# ab282865 |
|
| Cell line (Homo sapiens) |
HEK293T cells |
Cell Culture Facility, UC Berkeley |
N/A |
|
| Cell line (H. sapiens) |
HEK293-lenti-X cells |
Cell Culture Facility, UC Berkeley |
N/A |
|
| Cell line (H. sapiens) |
MDA-MB-231 cells |
Cell Culture Facility, UC Berkeley |
N/A |
|
| Cell line (H. sapiens) |
Hela cells |
Cell Culture Facility, UC Berkeley |
N/A |
|
| Cell line (H. sapiens) |
SH-SY5Y cells |
Cell Culture Facility, UC Berkeley |
N/A |
|
| Cell line (H. sapiens) |
U2OS cells |
Cell Culture Facility, UC Berkeley |
N/A |
|
| Cell line (H. sapiens) |
HEK293T-DNAJC5-CRISPR KO cells |
This study |
N/A |
|
| Cell line (H. sapiens) |
SH-SY5Y-DNAJC5-shRNA KD cells |
This study |
N/A |
|
| Cell line (Mus musculus) |
Mouse Embryonic Stem Cells |
|
|
|
| Cell line (M. musculus) |
Mouse Embryonic Stem Cells-hDNAJC5-OE cells |
This study |
N/A |
|
| Cell line (H. sapiens) |
Human-Induced Pluripotent Stem Cells |
University of Oxford; EBiSC repository |
N/A |
|
| Recombinant DNA reagent |
mCherry-Rab5CA (Q79L) |
Addgene |
Cat# 35138 |
|
| Recombinant DNA reagent |
BFP-Rab5CA (Q79L) |
This study |
N/A |
|
| Recombinant DNA reagent |
SNCA (Myc-DDK-tagged)-Human synuclein, alpha |
OriGene Technoogy |
Cat# RC221446 |
|
| Recombinant DNA reagent |
CSP (DNAJC5) (NM_025219) Human Tagged ORF Clone |
OriGene Technology |
Cat# RC208826 |
|
| Recombinant DNA reagent |
TCH1003-MGC premier cDNA clone for USP19 |
transOMIC |
Cat# TCH1003 |
|
| Recombinant DNA reagent |
pCMV-α-synuclein-A30P |
Gift of Dr. Thomas Südhof lab |
N/A |
|
| Recombinant DNA reagent |
pCMV-α-synuclein-E46K |
Gift of Dr. Thomas Südhof lab |
N/A |
|
| Recombinant DNA reagent |
pCMV-α-synuclein-A53T |
Gift of Dr. Thomas Südhof lab |
N/A |
|
| Recombinant DNA reagent |
αS-2 (α-syn-α-syn) tandem dimer |
Gift of Michael Woodside lab |
N/A |
|
| Recombinant DNA reagent |
αS-4 (α-syn-α-syn-α-syn-α-syn) tandem Tetramer |
Gift of Michael Woodside lab |
N/A |
|
| Recombinant DNA reagent |
pCMV-αS-2 |
This study |
N/A |
|
| Recombinant DNA reagent |
pCMV-αS-4 |
This study |
N/A |
|
| Recombinant DNA reagent |
pCMV-DNAJC5-L115R |
This study |
N/A |
|
| Recombinant DNA reagent |
pCMV-DNAJC5-L116Δ |
This study |
N/A |
|
| Recombinant DNA reagent |
pCMV-DNAJC5 (WT)-HaloTag |
This study |
N/A |
|
| Recombinant DNA reagent |
pCMV-DNAJC5 (L115R)-HaloTag |
This study |
N/A |
|
| Recombinant DNA reagent |
pCMV-XPACK-DNAJC5 (L115R)-HaloTag |
This study |
N/A |
|
| Recombinant DNA reagent |
mNeonGreen-α-synuclein |
This study |
N/A |
|
| Recombinant DNA reagent |
3H-α-synuclein |
This study |
N/A |
|
| Recombinant DNA reagent |
α-synuclein-3H |
This study |
N/A |
|
| Recombinant DNA reagent |
pCMV-XPACK-DNAJC5 (WT) |
This study |
N/A |
|
| Recombinant DNA reagent |
pCMV-XPACK-DNAJC5 (L115R) |
This study |
N/A |
|
| Recombinant DNA reagent |
pCMV-DEAD XPACK-DNAJC5 (L115R) |
This study |
N/A |
|
| Recombinant DNA reagent |
pCMV-DNAJC5-ΔJ (Δ14–82) |
This study |
N/A |
|
| Recombinant DNA reagent |
pCMV-DNAJC5-ΔC10 (D189–198) |
This study |
N/A |
|
| Recombinant DNA reagent |
pCMV-DNAJC5-ΔC20 (D179–198) |
This study |
N/A |
|
| Recombinant DNA reagent |
pCMV-DNAJC5-ΔC30 (D169–198) |
This study |
N/A |
|
| Recombinant DNA reagent |
pCMV-DNAJC5-ΔC40 (D151–198) |
This study |
N/A |
|
| Recombinant DNA reagent |
pCMV-pOTC-GFP |
This study |
N/A |
|
| Recombinant DNA reagent |
pCMV-pOTC-3H-GFP |
This study |
N/A |
|
| Recombinant DNA reagent |
pX330-Venus-DNAJC5-Exon 4-gRNA |
This study |
N/A |
|
| Recombinant DNA reagent |
pLenti-CMV-DNAJC5 |
This study |
N/A |
|
| Recombinant DNA reagent |
pLenti-CMV-DNAJC5 (L115R) |
This study |
N/A |
|
| Recombinant DNA reagent |
plKO.1-DNAJC5-ShRNA |
This study |
N/A |
|
| Software, algorithm |
Fiji (ImageJ) |
NIH |
https://imagej.nih.gov/ij/
|
|
| Software, algorithm |
PyMOL |
Schrödinger |
https://pymol.org/2/
|
|
| Software, algorithm |
Prism 8 |
Graphpad |
https://www.graphpad.com/scientific-software/prism/
|
|
| Software, algorithm |
AlphaFold Protein Structure Database |
DeepMind |
https://alphafold.ebi.ac.uk/
|
|
