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. 2022 Nov 30;613(7945):721–728. doi: 10.1038/s41586-022-05569-1

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 6 — (a): Synthetic transposon-insertion screen in ΔyghB V. cholerae. P-values were obtained by the Mann-Whitney U (MWU) test without adjustment for multiple comparisons. MFC: mean fold change in read coverage between ΔyghB and WT. (b): Growth of Δvca0040 V. cholerae expressing vectors with arabinose-inducible genes on regular LB (left), LB pH 9 (middle) and LB pH 9 + arabinose (right) plates. (c): Growth of V. cholerae lacking one or both of vca0040 and/or yghB on LB pH 6 plates. LB was buffered with 100 mM MES and pH-adjusted with HCl. a: pooled data from n = 2 independent transposon libraries. b, c: representative images from n = 3 cultures per strain/condition.