Fig. 2 – The distal hydroxyl (-OH) group of tyrosine PUFA analogues is necessary for robust I_Ks channel activation.

A) Structures of NAL-phe and Lin-phe. B) Representative current traces for NALT (gray), NAL-phe (black), Lin-tyr (pink), and Lin-phe with 0 μM PUFA (left) and 20 μM PUFA (right). C,E,G) I/I₀, E) ΔV_0.5, and G) G_max dose response curves for NAL-phe (n=4) and Lin-phe (n=4) with dotted lines representing dose response of NALT (n=4) and Lin-tyr (n=4). D,F,H) Maximum effects on D) I/I₀, F) ΔV_0.5, and H) G_max (at 20 μM) for NAL-phe (n=4), Lin-phe (n=4), NALT (n=4), and Lin-tyr (n=4). (Asterisks indicate statistically significant differences determined by one-way ANOVA with Tukey’s test for multiple comparisons.) Values for all compounds and concentrations available in Figure 2-source data 2.