Figure 4: Acquisition of C1498-derived K^b molecules by APC populations in the tumor. A-F).

K^b−/−D^b−/− mice were challenged with C1498 K^b+/+ (n = 7), K^b−/− (n = 17) or K^bAB (n = 19) cells. Tumors were analyzed at day 6–10 by surface K^b staining and flow cytometry. Gating strategy is depicted in (A). Representative histograms are shown for cancer cells (B), APCs (C), and T cells (D) isolated from the tumor. Data are quantified as MFI (E) or % K^b+ (F). G and H) ImageStream cytometry of CD11c⁺ MHC-II⁺ APCs from C1498.SIY (n = 1), C1498 K^b+/+ (n = 3) or K^bAB (n = 12) tumors in C57BL/6 hosts at day 6–10. Representative images are shown in (F), and quantification of intracellular versus surface K^b localization is shown in (G), with each bar representing one mouse. Statistical significance for (D) and (E) was determined by two-way ANOVA with post-hoc Tukey’s HSD test. Graphs are depicted as mean ± s.d. Data are pooled from five (A-E) or three (F-G) independent experiments. Also see Supplemental Figures S1–S3.