Dietary Advanced Glycation End-products Elicit Toxicological Effects by Disrupting Gut Microbiome and Immune Homeostasis

Abstract

The aging immune system is characterized by a low grade chronic systemic inflammatory state (“inflamm-aging”) marked by elevated serum levels of inflammatory molecules such as interleukin (IL)-6 and C-reactive proteins (CRP). These inflammatory markers were also reported to be strong predictors for the development/severity of Type 2 diabetes, obesity, and COVID-19. The levels of these markers have been positively-associated with those of advanced glycation end-products (AGE) generated via non-enzymatic glycation and oxidation of proteins and lipids during normal aging and metabolism. Based on the above observations, it is clinically-important to elucidate how dietary AGE modulate inflammation and might thus increase risk for aging- exacerbated diseases. The present narrative review discusses the potential pro-inflammatory properties of dietary AGE with a focus on the inflammatory mediators CRP, IL-6 and ferritin, and their relations to aging in general and Type 2 diabetes in particular. In addition, underlying mechanisms - including those related to gut microbiota and their receptors for AGE, and the roles AGE might play in affecting physiologies of the healthy elderly, obese individuals, and diabetics are discussed in regard to any greater susceptibility to COVID-19.

Introduction and methods

Advanced glycation end-products (AGE) are a class of heterogenous irreversible products primarily generated during the late stage of Maillard reactions, non-enzymatic glycation reactions between reducing sugars and amino acids/lipids/nucleic acids (Hodge 1953; Namiki and Hayashi 1983). AGE precursors can also be produced from oxidation of sugars (Dyer et al. 1991), lipids (Fu et al. 1996), and via polyol pathways, mainly following formation of interme- diary highly-reactive dicarbonyls. Among frequently-reported AGE (Figure 1) are N^ε-carboxymethyllysine (CML) (Ahmed et al. 1986), N^ε-carboxyethyllysine (CEL) (Ahmed et al. 1997), pentosidine (Miyata et al. 1996), argpyrimidine (Gomes et al. 2005), GOLD, MOLD or DOLD (lysine dimers crosslinked by two dicarbonyl molecules [glyoxal {GO}, methylglyoxal {MG} or 3DG, respectively]) (Nagaraj et al. 1996; Wells-Knecht et al. 1996), as well as GODIC, MODIC, and DODIC (arginine and lysine crosslinks) (Lederer and Klaiber 1999; Biemel et al. 2001). All these AGE are referenced by their core structures. As free amino acids, peptides, and proteins are involved in the crosslinking, the actual molecular weights (MWs) of AGE are highly diverse, and there is no clear separation between high and low MW AGE (Poulsen et al. 2013).

Figure 1.

Structures of common AGE. Modified from Akıllıoğlu and Gökmen (2019).

AGE are known for imparting detrimental effects on human health, in part because they accumulate in the extracellular matrix of various tissues; ultimately, such effects contribute to aging and chronic diseases (Kellow and Coughlan 2015). The modes of action by which AGE act in situ include: 1) crosslinking of proteins, lipids, and nucleic acids, leading to alterations in cell structures and functions; 2) activation of receptors for AGE, resulting in cell proliferation, autophagy, inflammation, and/or apoptosis; 3) generation of reactive oxygen species (ROS) that contribute to oxidative stress; and, 4) impairing mitochondrial function. Furthermore, some AGE can be recognized as antigens to induce immune responses. Dietary AGE are also known to possess allergenicity and immunogenicity properties that may play a role in food allergy (Gupta et al. 2018).

In this review, the immunotoxic characteristics of dietary AGE are reviewed in terms of pro-inflammatory potentials, with a focus on relationships with biomarkers of aging and Type 2 diabetes, i.e., C-reactive proteins (CRP), interleukin (IL)-6, ferritin, and overall lymphopenia. Cross-disciplinary approaches, including those in food science, toxicology, physiology, and immunology, have been used to critically-assess contributions of dietary AGE to disease progression through immune disruption. Potential underlying mechanisms of action for these AGE in a host, including changes induced in gut microbiota and their receptors for AGE that lead to aging-exacerbated diseases, are discussed here as well (Figure 2).

Figure 2.

Dietary advanced glycation end-products (AGE) induce toxicological effects by activating RAGE, modulating gut microbiota and inducing collagen crosslinking - potential roles in severity of COVID-19, aging, obesity and Type 2 diabetes. ADME = Absorption, distribution, metabolism, and excretion.

For this review, various databases including Google Scholar and PubMed were searched using terms such as aging, Type 2 diabetes, COVID-19, inflammation, advanced glycation end-products, and microbiome.

AGE in food

AGE can be generated both endogenously and exogenously. Food is a major exogenous source of AGE, especially those prepared under high-temperature conditions and stored for long periods or with food additives (Luevano-Contreras and Chapman-Novakofski 2010). AGE are naturally present in animal-derived foods, and cooking processes result in additional AGE formation (Uribarri et al. 2010). The absorption rate for dietary AGE is ≈ 10% in the human gastrointestinal (GI) tract; this correlates with AGE levels in circulation and tissues (Koschinsky et al. 1997). Due to heterogeneity in composition, various markers are used to quantify AGE in various specimen. The most commonly used marker for AGE is the non-fluorescent CML, because of their high abundance/wide distribution in biological (Reddy et al. 1995) and non-biological (Uribarri et al. 2010) samples. Fluorescence of AGE is another marker; however, not all AGE fluoresce and any fluorescence characteristics are specific to each individual AGE. Thus, any analytical readouts are highly-dependent on the composition of the AGE present and a combination of excitation/emission wavelengths applied (Schmitt et al. 2005).

Several studies have been conducted in attempts to establish a database for AGE. Most of the large-scale studies quantified CML levels using ELISA (Goldberg et al. 2004; Uribarri et al. 2010; Takeuchi et al. 2015) or LC-MS/MS (Hull et al. 2012; Scheijen et al. 2016). One study used ELISA to compare CML to glyceraldehyde, glucose, or fructose-derived AGE in a total of 1,650 beverages/foods commonly consumed in Japan (Takeuchi et al. 2015). Another study compared CML to two other markers of AGE, CEL and N^δ-(5-hydro-5-methyl-4-imidazolon-2-yl)-ornithine (MG-H1), in 190 foods using LC-MS/MS (Scheijen et al. 2016). In general, foods with high AGE content include nuts, biscuits, and cooked meat (Uribarri et al. 2010; Hull et al. 2012; Scheijen et al. 2016). Heating steps, such as occur in cooking and industrial processing, dramatically increase AGE levels in food. For example, the AGE level in beef was > 10-times higher after 4 min of grilling (Uribarri et al. 2010) and 5–10-times higher in evaporated semi-skimmed milk than in semi-skimmed milk (Scheijen et al. 2016). Kinetically, the Maillard reaction rate can be increased by 4–8-fold/10°C (Kaanane and Labuza 1989). In comparison, fruits and vegetables are low in AGE (Uribarri et al. 2010). Lastly, most beverages are low in CML, CEL, or MG-H1 (Uribarri et al. 2010; Takeuchi et al. 2015; Scheijen et al. 2016), but high in fructose- and glucose-derived AGE (especially the latter).

Large discrepancies have been found regarding AGE content in high-fat foods and cereals when using different quantifying methodologies. For example, higher AGE levels were detected in fatty foods by ELISA, and in cereals by LC-MS/MS (Uribarri et al. 2010; Hull et al. 2012; Scheijen et al. 2016). One study compared GC-MS and ELISA approaches in the quantitation of CML levels in three types of milk samples: (1) powdered infant formula, (2) milk consisting of whey protein isolate (WPI), lactose and ascorbate, and (3) hydrolyzed liquid infant formula. The two methods correlated well for powdered infant formula (r² = 0.966) and milk consisting of WPI, lactose, and ascorbate (r² = 0.996), although a higher CML level was detected in the powdered formula with the ELISA. In contrast, no satisfactory correlation was obtained for hydrolyzed liquid infant formula, with a much higher CML detection when ELISA was used (Charissou et al. 2007). This might be explained by an unspecific interference of ELISA by the lipid matrix, which could account for the overestimation of AGE in fatty foods by ELISA in general. Another study quantitated CML levels in gruel samples by ELISA and ESI-LC-MS/MS: average CML levels measured by ELISA was 54% of that measured by ESI-LC-MS/MS (Tareke et al. 2013), suggesting under-estimation of AGE levels in cereal by quantitating CML using ELISA. Using UPLC-MS, CML levels in cereals were identified as low; however, the intake of cold breakfast cereals could lead to elevated serum and urinary CML levels in adults (Semba et al. 2012). A “fructositis” hypothesis has been proposed to explain this phenomenon: high fructose-to-glucose ratios promotes the intestinal in situ formation of fructose-associated AGE (de Christopher 2017).

Absorption, distribution, metabolism, and excretion (ADME) of dietary AGE

Exposure to dietary AGE is dependent on eating habits and age, since AGE content varies in different foods. A Western diet (WD) high in processed and red meats, high-fat dairy, refined grains, sweets and desserts, contains much higher AGE levels than a prudent diet high in fruits, vegetable, fish, legumes, whole grains) (Lopez-Garcia et al. 2004). A 70-kg adult fed a WD is estimated to take in 1 mg CML/kg body weight (BW) daily, while a 6-kg infant takes in > 2.5 mg CML/kg BW/day through consumption of 1 L infant formula (Delgado-Andrade et al. 2007; Hull et al. 2012; van Rooijen et al. 2014). Breastfeeding significantly reduces levels of AGE in infants compared to in those who are formula-fed (Federico et al. 2016).

It was estimated ≈ 10% of dietary AGE can be absorbed after oral ingestion and then transported into circulation, with two-thirds of these AGE remaining in the body (Koschinsky et al. 1997). Due to this low absorption rate, pathological effects of dietary AGE have been mostly neglected, even though both human and animal studies have shown dietary AGE can contribute to the pool of AGE in a body. In a cohort of 450 participants, uptake of high-dietary AGE resulted in an elevation of free CML, CEL, and MG-H1 levels in plasma and urine, but not in the protein-bound forms (Scheijen et al. 2018), suggesting protein-bound AGE likely arise endogenously. Another study of 90 healthy people showed a reduction of dietary intake of AGE was associated with an average 30–40% decrease in serum AGE levels (Uribarri et al. 2005). Exposure to heat-treated (200°C, 10 min) high-fat diet by male ApoE^−/− mice for 8 wk produced increases in plasma CML and CEL levels and in spleen weight when compared to values in mice fed a control high-fat diet (Marungruang et al. 2016).

Interestingly, there seemed to be a threshold for dietary AGE to have an effect on AGE levels in a body. It was seen that dietary consumption of AGE at levels < 0.5 × 10⁶ U would not result in increases in serum AGE levels. Once the threshold was reached, a significant correlation (r² = 0.8, p < 0.05) was found between the amounts of AGE ingested and resultant elevations in serum AGE kinetics (Koschinsky et al. 1997). This plateau phenomenon for serum AGE has also been observed in mice. C57BL/6 male mice fed chow containing 323 ng CML/g had no detectable CML in their sera. However, further oral administration of WPI-glucose-derived AGE at a dose equivalent to the amount of CML the mouse received in the diet produced an average serum CML level of 150 ng/ml (Chen and Guo 2018). In another mouse study, serum CML levels almost doubled when dietary CML intake increased from 16.0 × 10⁴ - 24.4 × 10⁴ U/day, and remained at that level even when dietary CML intake was 30 × 10⁴ U/day (Cai et al. 2012).

It was reported in animal studies that oral AGE exposure was associated with increased AGE levels in the kidney, liver, lung, heart, tendons (Roncero-Ramos et al. 2013, 2014; Li et al. 2015), and GI tract (Yuan et al. 2018). A limitation with those studies was that they were unable to differentiate if increases in tissue AGE levels were directly a result of deposition of exogenous AGE or indirectly from boosted accumulation of endogenous AGE. One study used dietary protein-bound [¹³C]-labeled CML that directly traced distribution of dietary AGE to discriminate it from endogenous AGE. After chronic oral exposure, the [¹³C]-CML was directly deposited in organs, with high levels found in the kidney, ileum, colon and lung; the material was found at > 10-times lower levels in the brain, testis, heart, skeletal muscle, liver, and fat. Moreover, an intake of CML that was ≈ 10 times higher than the dietary level increased endogenous CML levels in the colon (almost doubled) and muscles, but not in other organs (Tessier et al. 2016).

The fate of ingested AGE is under extensive investigation; there are many reports available on AGE deposition and distribution in organs and tissues (Figure 2). A human study showed that 1/3 of absorbed AGE was secreted into the urine within 48 hr (Koschinsky et al. 1997). Another study found urinary CML secretion was related to the forms and complexity of CML, i.e., high MW and insoluble fractions from bread crust extractions decreased urinary secretion rates compared to whole bread crust extraction (Roncero-Ramos et al. 2013); this was due to the anti-digestive properties of insoluble protein-bound CML. Fecal excretion is another major route for AGE disposition, i.e., ≈ 1/3 of dietary AGE eliminated based on CML quantitation (Roncero-Ramos et al. 2013). This quantity might be under-estimated because part of the dietary AGE was likely degraded to low MW compounds by gut microbiota (Tuohy et al. 2006). Also, a small portion of serum CML can be passed into breast milk (Dittrich et al. 2006).

In terms of metabolism, AGE are not typical substrates for detoxifying Phase 1 and 2 enzymes (Poulsen et al. 2013). Small endogenously-formed glycated and misfolded proteins are targets for intracellular degradation by the ubiquitin-proteasome-system 20S proteasome (Jung et al. 2009). Large bulky glycated proteins can also form after oxidation and cross-linking. If not eliminated by the lysosomal system, they can accumulate in cells and tissues (Teodorowicz et al. 2018).

Mechanisms of immunotoxicity following dietary exposure to AGE

AGE are considered immunotoxicants as part of their overall toxicologic profile (Kellow and Coughlan 2015). The main effects of AGE on immunity are to induce pro-inflammatory responses. In the current review, two mechanisms, including regulation of receptor for AGE (RAGE) and gut microbiota, are discussed to illustrate how dietary AGE induce immunotoxicity (Figure 2). Other mechanisms have also been reported. For example, the AGE receptor 1 (AGER1, responsible for endocytic uptake and degradation of AGE) can suppress RAGE expression and negatively-regulate any oxidative stress and inflammation induced by the AGE (Lu et al. 2004; Ott et al. 2014). Consumption of dietary AGE can deplete AGER1 in adipocytes, resulting in increases in inflammation, oxidative stress, and insulin resistance (Cai et al. 2012).

Receptor for AGE

RAGE (a 35 kD transmembrane receptor of immunoglobulin superfamily; Neeper et al. 1992) is expressed on a range of cell types, including immune cells (Ott et al. 2014). RAGE plays an important role in inflammatory processes and endothelial activation. In vitro application of AGE induces inflammatory responses in macrophages (Jin et al. 2015; van der Lugt et al. 2018) and promotes differentiation of native CD4⁺ T-cells toward a pro-inflammatory status by its binding to RAGE (Han et al. 2014). Up-regulation of RAGE expression in different organs and tissues has been observed in rodents on diets/drinking water containing AGE/MG (Cai et al. 2012; Sena et al. 2012). Activation of RAGE results in intracellular ROS production (Coughlan et al., 2009) and activation of p21(ras)-dependent mitogen-activated protein kinase (MAPK) pathways (Lander et al. 1997), which eventually lead to up-regulation of NF-κB and inflammation (Figure 3). The consequent elevations in circulating levels of cytokines such as IL-1, IL-6 and tumor necrosis factor (TNF)-α ultimately will support a persistent state of inflammation. In a study that investigated effects of various AGE (BSA + D-glyceraldehyde, BSA + D-glycolaldehyde, BSA + MG, BSA + GO) on monocyte expression of adhesion molecules, interferon (IFN)-γ and TNF-α production, and T-cell proliferation, it was found that the effect of AGE on immune cells depended on the AGE subtype present (Ohashi et al. 2010).

Figure 3.

Schematic representation of paradoxical control of pro- and anti-inflammation by RAGE activation.

In contrast, it was reported that mixed and purified Maillard reaction products (MRP) containing AGE imparted anti-oxidative and anti-inflammatory effects when applied to human Caco-2 epithelial colorectal adenocarcinoma cells (Chen and Kitts 2011; Kitts et al. 2012). It was also shown that ribose-tryptophan MRP had anti-inflammatory effects in the lipopolysaccharide (LPS)-treated murine macrophage RAW 264.7 cell line (Qin et al. 2018). Those investigators identified one anti-inflammatory ribose-tryptophan MRP as 532.24 Da 3-((1H-indol-3-yl)-methyl)-8-(5-((1H-indol-3-yl)methyl)-6-oxomorpholin-2-yl)-9-hydroxy-1,7,4-dioxazecan-2-one (Qin et al. 2018). In another study, Huang et al. (2015) found that AGE attenuated nitric oxide effects on human renal tubular cells via RAGE-JAK2-STAT1/STAT3 activation and consequent SOCS-3 suppression. These apparently contradictory findings to the main literature reflect the fact that binding of RAGE ligands may not only lead to pro- but to anti-inflammation as well (Figure 3). Treatment of THP-1 macrophage cells with 1 μg/ml high mobility group box (HMGB) 1 (RAGE ligand) polarized the cells to an anti-inflammatory M2 state; this too was via impact on RAGE-SHIP/SOCS1 (Rojas et al. 2016). The dual roles of RAGE in a pro-\anti-inflammatory balance seems dose-related; however, this needs further study. Interestingly, anti-inflammatory “functions” of AGE can be RAGE-independent. For example, BSA-glucose-derived AGE suppressed LPS-induced M1 polarization of bone marrow-derived macrophages, and the effect was due to a dampening of NLRP3 inflammasome assembly (Son et al. 2017).

Another possibility could be some MRP (other than AGE) in the above-tested mixtures imparted anti-inflammatory effects. Studies have shown WPI-glucose-derived early glycation products (EGP; Figure 4A) and AGE differentially-modulated host macrophage cytokine/chemokine profiles (Chen et al. 2018; Chen and Guo 2018). AGE induced inflammation (consistent with a majority of reports) and EGP were anti-inflammatory (Chen et al. 2018). The latter effect was further evidenced by a dramatic elevation in serum IL-10 levels (Figure 4B) and enhanced M2 polarization in C57BL/6 male mice with prostate tumors orally-administered EGP (Chen and Guo 2018). Though the inflammatory responses induced by WPI-glucose-derived AGE were conceivably via RAGE, anti-inflammatory responses induced by EGP could be using a different mechanism. One study found that EGP at levels up to 10 mg/ml were unable to induce RAGE expression in human macrophages (unpublished observation). In addition to RAGE, AGE can also interact with scavenger receptors predominantly involved in capture, removal, and degradation of AGE. This group includes Type I and Type II macrophage scavenger receptors, CD36, FEEL-1 and FEEL-2, SR-BI and SR-BII, and Lox-1 (Byun et al. 2017). Thus, EGP might bind one of these receptors to initiate effects.

Figure 4.

Differential immunomodulating capabilities of EGP and AGE. (A) Structure of glucose-derived Amadori compounds (early glycation products). R = protein/peptide/amino acid. (B) Comparison of EGP and AGE in modulating cytokine/chemokine production. Modified from Chen and Guo (2018). ^NoNo significant change when compared to non-reacted control. ^aValue significantly different from EGP but not from non-reacted control.

Gut microbiota

The microbiota plays a major role in inflammation because of its tight relationship with immune system development and maturation. Many dietary AGE have high MW and are not absorbed in the intestine; instead, they pass through the GI to the colon and are potentially metabolized by gut microbiota (GMB). Thus, it is not surprising to see an increasing body of literature that shows that dietary AGE can induce gut dysbiosis, e.g., a GMB imbalance (Table 1).

Table 1.

Summary of human and lab animal microbiome studies on Maillard reaction products (AGE and EGP).

Disease Model	Population/Animal Model	Exposure Windows	MRP/Dose/Concentration	Routes of Administration	Diet	Effects	Reference
Autoimmune prostatitis	Male NOD mice	Feeding (6 mo) starting at ≈ 4-mo-of-age	600 mg EGP/kg	Gavage	Diet 5053, PicoLab	Increased Anaerostipes, Parabacteroides, Prevotella, Allobaculum and Bacteroides and decreased Adlercreutzia and Roseburia in terms of relative abundance.	Chen, et al. 2019
Not Specified	Weanling Wistar rats	Feeding for 88 d	Diets containing bread crust or its soluble high molecular weight, soluble low molecular weight or insoluble fractions	In food	AIN-93G purified diet	Low and high MW fractions rich in Amadori compounds down-regulated Lactobacillus spp.; the insoluble fraction abundant in HMF and CML down-regulated E. rectale/C. coccoides and C. leptum	Delgado-Andrade et al. 2017
Not Specified	Sprague-Dawley rats	Feeding for 2 wk	High in furosine (Amadori compound-derived marker for initial stage of Maillard reaction), CML, and CEL	In food		Increases in Akkermansia, Allobaculum and Lachnospiraceae_UCG-006, and a decrease in Erysipelatoclostridium at genus level compared to in hosts fed heated control	Han et al. 2018
Atherosclerosis	Male ApoE−/− mice	Feeding (8 wk) starting at 8-wk-of-age	Plasma CML and CEL increased 1.7- and 2.5-fold, respectively	In food	Heat-treated high-fat diet	Decreased α diversity accompanied by increases in Allobaculum and unclassified genus of Clostridiales and decreases in Bacteroides, unclassified genera of Lachnospiraceae, Rikenellaceae and Ruminococcaceae at genus level	Marungruang et al. 2016
Inflammatory bowel diseases	Male BALB/c mice	Feeding (3 wk) starting at 7-wk-of-age	Nε-Carboxymethyllysine; 1.6 mg/kg/day	Per os	Standard chow	Bacteroidaceae increased, Lachnospraceae decreased	ALJahdali et al. 2017
Not Specified	Male Sprague-Dawley rats	Fed for 6, 12, or 18 wk	Fluorescent AGE (968 v. 2148 AU/g), CML (272 v. 143 μg/g), CEL (6.26 v. 0.97 μg/g), GO (49.1 v. 12.1 mg/kg), and MGO (28.7 v. 1.1 mg/kg) were higher in the H-AGE diet	In food	AIN-93G diet enriched with AGEs	Decreased α diversity, Alloprevotella and Ruminococcaceae, while increasing Allobaculum and Bacteroides	Qu et al. 2017
Not Specified	Male C57BL/6 mice	Feeding (8 mo) starting at 6-wk-of-age	Same as above	In food	Same as above	Decreased α diversity, increased Alloprevotella, Helicobacter, Parabacteroides, Ruminococcaceae_UCG-014 and unclassified genus of Rhodospirillaceae, and decreased Alistipes, Desulfovibrio, Lachnospiraceae_NK4A136_group and Rikenellaceae_RC9_gut group.	Qu et al. 2018
Not Specified	Adolescent men	2-wk randomized two-period crossover trial	Diets high or low in hydroxy-methylfurfural (HMF; 5-fold) and CML (2-fold)	In food	Prepared by a local catering firm	Negative correlations between Lactobacilli numbers and dietary advanced MRP (e.g., AGE); Bifidobacteria counts negatively correlated with Amadori compound intake (e.g., EGP).	Seiquer et al. 2014
Not Specified	Male weanling Wister rats	Feeding (87 days) starting at weanling	High in Amadori compounds, HMF and CML	In food	AIN 93G diet	Total bacteria and Lactobacilli were negatively-correlated with MRP intake; no correlations were found with Bifidobacteria.	Seiquer et al. 2014
End stage renal disease (ESRD) patients	Undergoing peritoneal dialysis	One-month dietary AGE restriction	Dietary AGE restriction resulted in decreases in serum CML (29.6 vs. 23.3 u/ml) and methylglyoxal-derivatives (5.6 vs. 4.0)	In food	Habitually consuming a high AGE diet	Dietary AGE restriction significantly decreased Prevotella copri and Bifidobacterium animalis relative Abundance, and increased Alistipes indistinctus, Clostridium citroniae, Clostridium hathewayi and Ruminococcus gauvreauii relative abundance	Yacoub et al. 2017

In male BALB/c mice orally-exposed to CML, Bacteroidaceae levels were increased while those of Lachnospraceae decreased (Al Jahdali et al. 2017). In male ApoE^−/− mice, there were increases in plasma CML and CEL levels after host exposures to heat-treated high-fat diets; this was accompanied by a decreased α diversity (increases in Allobaculum and unclassified genus of Clostridiales and decreases in Bacteroides, unclassified genera of Lachnospiraceae, Rikenellaceae and Ruminococcaceae at genus level) (Marungruang et al. 2016). Decreased α diversity was also seen in male C57BL/6 mice exposed to an AIN-93G diet enriched with AGE; these hosts manifest increased gut levels of Alloprevotella, Helicobacter, Parabacteroides, Ruminococcaceae_UCG-014 and unclassified genus of Rhodospirillaceae, and decreased Alistipes, Desulfovibrio, Lachnospiraceae_NK4A136_group, and Rikenellaceae_RC9_ gut group levels. The diet with high AGE levels also altered fecal short-chain fatty acid (SCFA) levels in the hosts, e.g., increasing isobutyrate and isovalerate while decreasing acetate and butyrate levels (Qu et al. 2018). The same high-AGE diet also decreased α diversity (i.e., decreased Alloprevotella and Ruminococcaceae, while increased Allobaculum and Bacteroides) in male Sprague-Dawley rats (Qu et al. 2017). The ammonia concentration in the rat cecal contents was increased, while that of acetate concentration was decreased. In addition, increased epithelial damage and lymphocyte infiltration, and decreased tight junction in the colon were noted.

One conclusion reached based on all the above data is that AGE consumption decreases GMB species richness - based on consistent patterns of decreased α diversity. As for individual bacteria, no broad conclusions can yet be drawn due to inconsistencies among the animal studies. However, Alistipes (decreased in mice by a diet containing high levels of AGE [Qu et al. 2018]), was found at a lower abundance in the gut of peritoneal dialysis patients (Yacoub et al. 2017) who had higher serum AGE levels (Table 1) and in patients with non-alcoholic fatty liver disease (Jiang et al. 2015). In addition, gut levels of some strains of Helicobacter were augmented by increases in AGE (Qu et al. 2018).

In contrast, some GMB changes induced by AGE seem to be beneficial. For example, colonization/prevalence of several gut inflammation-inducing strains from the Desulfovibrio genus were decreased by consumption of diets containing high AGE levels (Loubinoux et al. 2002; Figliuolo et al., 2017; Qu et al. 2018). Increasing levels of Allobaculum due to consumption of an AGE-rich diet by ApoE^−/− mice (Marungruang et al. 2016) and Sprague-Dawley rats (Qu et al. 2017) were suggested to be beneficial to maintaining a healthy colon mucus layer and a reduced overall inflammatory status (Jakobsson et al. 2015). Interestingly, there is one report to show that oral CML exposure alleviated gut dysbiosis induced by dextran sulfate sodium salt, but not by trinitro-benzenesulfonic acid, in colitic mice (Al Jahadali et al. 2017).

As discussed above, the disagreement between AGE-induced beneficial and detrimental changes in GMB could partially be due to the composition and abundance of MRP in the test diet or samples (Snelson and Coughlan 2019). Two experiments were conducted in which adolescent male humans consumed diets that were either high or low in hydroxymethylfurfural (HMF, an MRP generated in intermediate stage) and CML, and male weanling Wister rats fed diets with or without a glucose-lysine mixture high in Amadori compounds, HMF and CML (Seiquer et al. 2014). No significant differences were detected for plasma biochemical\anthropometric parameters in either experiment; however, discrepancies between the two studies occurred in trying to correlate GMB and MRP markers as further discussed below. In the human study, negative correlations were found between Lactobacilli numbers and dietary advanced MRP (e.g., AGE), whereas Bifidobacteria counts were negatively-correlated with Amadori compound intake (e.g., EGP). In the rats, total bacteria and Lactobacilli levels negatively correlated with MRP intake, and no correlations were found for Bifidobacteria (Table 1). The authors concluded specific effects of dietary MRP were likely due to dietary amounts of the different browning compounds with distinct chemical structures. This notion was verified in a study using different fractions isolated from bread crust to feed weanling rats (Delgado-Andrade et al. 2017). Low and high MW fractions rich in Amadori compounds were found to up-regulate total levels of gut SCFAs, formic, and propionic acid, while the same agents down-regulated gut Lactobacillus spp levels. It was also seen that the insoluble fraction abundant in HMF and CML up-regulated gut formic and acetic acid levels, while down-regulating gut E. rectale/C. coccoides and C. leptum levels.

Effects of Amadori compounds on GMB have been explored. When exposed to a diet high in furosine (Amadori compound-derived marker for initial stage of Maillard reaction), CML and CEL, Sprague-Dawley rats (when compared to counterparts fed a heated-control diet) had decreased colonic levels of inflammatory TNFα and IL-6, and altered GMB (increased Akkermansia, Allobaculum and Lachnospiraceae_UCG-006, and decreased Erysipelatoclostridium at genus level). In addition, these rats displayed normal colons with only some decreases in crypt depth (Han et al. 2018). These likely beneficial effects on gut health suggested a regulatory effect for Amadori compounds on GMB and anti-inflammatory responses.

In recent studies, aged male non-obese diabetic (NOD) mice were treated by gavage with EGP that contained only Amadori compounds generated from a WPI-glucose system (Chen et al. 2019). These EGP-treated mice had an increased survival rate and decreased inflammation and immune infiltration into their prostatic lobes (Chen et al. 2020). When the microbial taxa at the genus level were compared, EGP treatment led to increases in gut levels of Anaerostipes, Parabacteroides, Prevotella, Allobaculum and Bacteroides, but decreases in Adlercreutzia and Roseburia (in terms of relative abundance; Table 1). The up-regulated Bacteroides acidifaciens was correlated with most of the immune parameters measured in the rats. Anaerostipes spp. express enzymes required for production of butyrate that protects NOD mice against diabetes (Mariño et al. 2017), and it is associated with a reduction of plasma glucose, insulin resistance and body weight in diabetic mice fed with high-fat diet (Xu et al. 2018). Bacteroides acidifaciens is important for promoting IgA production in the large intestine, and it is a potential treatment for metabolic diseases like obesity (Yanagibashi et al. 2013). Overall, EGP-treated mice exhibited a healthier GMB than that of the controls.

Toxicological effects of dietary AGE on diseases through immune disruption

Formation of AGE takes place as a part of normal aging and metabolism, and occurs at an accelerated rate in hyperglycemic, inflammatory and oxidative stress conditions. In this section, the toxicological effects of dietary AGE on aging and Type 2 diabetes in relation to RAGE and gut dysbiosis are discussed (Figure 2).

Aging

A substantial body of evidence shows that AGE and their functionally-compromised adducts are linked to, and perhaps responsible for, changes seen in the function of cells and tissues during aging, and then in development of many age-related morbidities, e.g., atherosclerosis, nephropathies, retinopathy, osteoarthritis, neurodegenerative diseases, diabetes mellitus (Ott et al. 2014; Spauwen et al. 2015; Drenth et al. 2018). High levels of circulating AGE can be used to predict cardiovascular disease mortality among older community-dwelling women (Semba et al. 2009). Administration of aminoguanidine (inhibitor of AGE formation) for 24–30 wk in normotensive WAG/Rij rats prevented age-related cardiac hypertrophy and arterial stiffness (Corman et al. 1998). Similarly, a presence of AGE was also associated with motor function decline in aging, and it was speculated that high levels of AGE may be a biomarker for low physical activity (Drenth et al. 2018). In a study of 559 moderate-to-severely disabled women (age 65 and older), women with higher CML concentrations had less grip strength than those with lower CML; from this it was concluded that women with higher AGE have more muscle weakness (Dalal et al. 2009). Interestingly, brain tissues of Alzheimer’s disease patients were found to contain higher AGE levels than brains of age-matched controls (Cruz-Sánchez et al. 2010).

It is likely that increases in endogenous production and exogenous intake, and lower clearance and detoxification, lead to accumulation of AGE in older populations. However, higher AGE levels occur in both healthy older adults and those with chronic diseases. Studies have/are being tried to identify mechanisms to explain why some human tissues are damaged while others are not in those states. One mechanism involves increased crosslinking within collagen and the extracellular matrix with age-related increases in AGE levels (Sims et al. 1996). Glycated low-density lipoproteins can crosslink with collagen to prevent uptake by cell receptors. These modified low-density lipoproteins are instead more likely phagocytosed by macrophages to form foam cells and, ultimately, development of atheroma (Bucala et al. 1994). Tissue accumulation of AGE can be further enhanced by some cardiovascular changes associated with aging, such as vascular stiffening, diastolic dysfunction, and endothelial dysfunction (Fishman et al. 2018).

The aging immune system is characterized by a low grade chronic systemic inflammatory state (“inflamm-aging”) marked by elevated inflammatory molecules, such as IL-6, CRP, ferritin, and lymphopenia (Dennis et al. 1998; Li et al. 2011; Cankurtaran et al. 2012). In hemodialysis patients, tissue levels of AGE are an independent determinant of CRP levels (Nagano et al. 2011). Though CRP is an acute-phase protein of hepatic origin, AGE cannot directly stimulate hepatocytes to produce CRP, but they enhance its expression by stimulating monocytes/macrophages to produce cytokines like IL-6 (Li et al. 2007). Circulating AGE levels correlate with those for IL-6 and other inflammatory markers in rheumatoid arthritis (Hein et al. 2005). In gingival fibroblasts, AGE also increase IL-6 expression (Nonaka et al. 2018). In a group of elderly patients with mild cognitive impairment, serum RAGE levels positively-correlated with both AGE and CRP levels (Gorska-Ciebiada et al. 2015). Further, CRP can up-regulate RAGE expression in endothelial cells (Zhong et al. 2006) and in THP-1 cells (Mahajan et al. 2010). Thus, AGE may contribute to the aging processes through exacerbating “inflamm-aging”.

Ferritin is a major tissue iron-storage protein that exhibits a variety of activities relevant to the immune system, including binding to T-cells, suppressing delayed-type hypersensitivity reactions (to induce anergy), suppressing B-cell antibody production, reducing phagocytosis by granulocytes, and regulating granulo-monocytopoietic processes (Zandman-Goddard and Shoenfeld 2008). Ferritin level increases with aging as a part of “inflamm-aging” (Cankurtaran et al. 2012). Macrophages accumulate ferritin during inflammation and polarization to pro-inflammatory M1. In β-thalassemia patients, circulating ferritin levels were seen to positively-correlate with levels of pentosidine, a fluorescent protein crosslink used as a biomarker for AGE (Mirlohi et al. 2018).

Studies in animals have suggested GMB alterations might cause aging. The GMB from old mice contribute to “inflamm-aging” after fecal microbiota transplantation to young germ-free mice (Fransen et al. 2017). Work with African turquoise killifish has shown that acute transfer of GMB from young donors to antibiotic-treated middle-age recipients extends life-span and delays behavioral aging (Smith et al. 2017). The elderly have a different GMB profile when compared to healthy adults. Generally, the diversity of GMB and abundance of commensals that maintain immune tolerance in the gut are reduced, while that of opportunistic pathogens that stimulate gut inflammation are increased (Nagpal et al. 2018) - this is somewhat consistent with gut dysbiosis induced by AGE [discussed earlier]. Aging generally leads to chronic systemic inflammatory states with hyperactive innate immune responses, particularly in the form of elevated neutrophil (PMN) accumulation following respiratory infection (Chen et al. 2020). Depletion of GMB using antibiotics significantly reduces levels of circulating aged neutrophils (Zhang et al. 2015).

It is of note that older people have greater susceptibility to the severe acute respiratory syndrome coronavirus 2 (SARS-CoV2; COVID-19), and that COVID-19 patients have increased circulating PMN-to-lymphocyte ratios (Liu et al. 2020). Adverse effects of AGE on PMN are well-known, including inhibited bacterial killing (Collison et al. 2002), suppressed migration (Toure et al. 2008), and induction of both oxidative stress and respiratory burst (Wong et al. 2003; Bansal et al. 2012). AGE also impact promotion of CD4⁺ T cell differentiation to pro-inflammatory states (Lu et al. 2019). Thus, it is likely modulation of innate immunity induced by AGE contributes to the observed “age-enhanced” mortality to SARS-CoV-2 infection. It is possible these outcomes are mediated in part through AGE-induced alterations in the host GMB.

Type 2 diabetes (T2D)

T2D, which accounts for ≈ 90% of all diabetes cases, is characterized by insufficient secretion of insulin from pancreatic β-islet cells, coupled with impaired insulin actions in target tissues such as muscle, liver, and fat (termed insulin resistance). Approximately one-third of U.S. adults > 65 year-of-age have T2D, and an additional one-third of older adults have pre-diabetes (Cowie et al. 2009). Serum or plasma AGE levels are generally elevated in T2D patients due to hyperglycemia (Vlassara et al. 2002). RAGE was also dramatically up-regulated in T2D patients (Yan et al. 2009). Hyperglycemia increases glycation processes in insulin-independent tissues and cells (like red blood cells, peripheral nerves, endothelial cells, eye lenses, and kidneys) (Tessier 2010). In vitro, pancreatic β-cells exposed to AGE displayed insulin secretory defects; in vivo, islets were damaged in Sprague-Dawley rats after chronic intraperitoneal injections of AGE in the form of modified rat serum albumin (Coughlan et al. 2011).

The immune system plays an important role in controlling whole-body metabolism and contributes significantly to the pathogenesis of T2D (Tsalamandris et al. 2019). Intake of AGE in the diet increases levels of inflammatory mediators (i.e., CRP, TNF-α, VCAM-1) found in the sera of T2D patients (Vlassara et al. 2002), and the increased serum AGE level is related to a rapid development of diabetic complications (Zheng et al. 2002; Rhee and Kim, 2018). For example, AGE have also been implicated in a delayed wound healing in T2D patients (Peppa et al. 2009). In elderly T2D patients with mild cognitive impairment, serum AGE, RAGE and CRP levels were increased (Gorska-Ciebiada et al. 2015). On the other hand, Vlassara et al. (2002) noted that reduced intake of AGE in T2D patients contributed to decreased levels of circulating AGE and inflammatory markers like TNFα and CRP. Another study suggested blood IL-6 and AGE levels were significant independent determinants of CRP in diabetics (Tan et al. 2004).

T2D is frequently associated with elevated levels of serum ferritin (Lecube et al. 2004). T2D is also associated with intestinal dysbiosis. Among the commonly reported findings, the genera of Bifidobacterium, Bacteroides, Faecalibacterium, Akkermansia and Roseburia were negatively associated with T2D, while the genera of Ruminococcus, Fusobacterium, and Blautia were positively-associated with T2D (Gurung et al. 2020).

COVID-19

While this review was in preparation, various investigators have hypothesized a role for the RAGE axis in COVID-19 pathogenesis (Kerkeni and Gharbi 2020; Stilhano et al. 2020), as well as in diabetes (de Francesco et al. 2020) and lung inflammation (Andersson et al. 2020; Rojas et al. 2020). Unfortunately, at present, there do not appear to be any studies specifically tackling the topic of dietary AGE and any potential contribution to COVID-19 morbidity. It is worth noting a recent spike in studies surrounding soluble RAGE measures in COVID patients (see Dozio et al. 2020; Lim et al. 2021). AGE may contribute to organ damage by promoting host cell death (Mao et al. 2018). Importantly, the levels of soluble RAGE in bronchoalveolar lavage fluid - which reflect tissue RAGE expression (Nakamura et al. 2007) - were found to correlate with severity of various inflammatory lung diseases (Uchida et al. 2006; Kamo et al. 2015; Stockley et al. 2019). Thus, it seems it would be clinically-important to elucidate if AGE help to exacerbate inflammation, and by doing so increase the risk for COVID-19 development and severity in susceptible populations.

Conclusions

In this review, dietary sources, ADME, immunotoxic effects, and underlying mechanisms of action by AGE were discussed. Dietary AGE are an important exogenous source of AGE and may contribute to an AGE pool in a body. Some studies indicated effects of AGE are subtype-dependent. Most studied AGE were mixtures generated in reactions between BSA and glucoses. Even with the same reactants, the composition/abundance of each component of AGE can vary; these are often primarily determined by the incubation conditions (e.g., time, pH, temperature, reactant ratio). A complete reaction leads to the production of melanoidins, while an incomplete reaction results in the generation of EGP in the initial or intermediate stages. Mixture impurity could also affect assay outcomes. Therefore, further identification and purification of functional AGE would be a strategy to permit stronger conclusions to be reached. Nonetheless, elevated serum and organ levels of AGE can induce chronic inflammation and contribute to the progression of various diseases, including aging, Type 2 diabetes and possibly COVID-19.

Abbreviations

ACE

angiotensin converting enzyme

ADME

absorption, distribution, metabolism, and excretion

AGE

advanced glycation end-product

AGER1

AGE receptor-1

CEL

N^ε-carboxyethyllysine

CML

N^ε-carboxymethyllysine

COPD

chronic obstructive pulmonary disease

CRP

C-reactive proteins

EGP

early glycation products

GMB

gut microbiota

HMGB

high mobility group box

HMF

hydroxymethylfurfural

MG

methylglyoxal

MG-H1

N^δ-(5-hydro-5-methyl-4-imidazolon-2-yl)-ornithine

MRP

Maillard reaction products

NOD

non-obese diabetic

PMN

polymorphonuclear neutrophil

RAGE

receptor for AGE

SCFA

short chain fatty acid

T1D

Type 1 diabetes

WPI

whey protein isolate

3-DG

3-deoxyglucosone

Biographies

Yingjia Chen was graduate student in University of Georgia Interdisciplinary Toxicology Program and College of Veterinary Medicine.

Tai L. Guo is Full Professor in the UGA College of Veterinary Medicine, with a specialty in endocrinology and immunotoxicology.