Fig. 4. scRNA-seq analysis of a soft-tissue lesion in the lower limb six months after anti-BCMA CAR T-cell therapy showed expression of epithelial-associated genes in multiple myeloma cells.

A UMAP embedding of 16,007 single cells from an extramedullary soft-tissue lesion colored by cell type. B Dotplot showing scaled expression (color) of respective marker genes per cell type. Dot size depicts the percentage of non-zero expressing cells. C Log-normalized gene expression of CXCR4, the CAR T target antigen TNFRSF17 (BCMA), proliferation marker MKI67, CCND2 (marking malignancy), as well as epithelial cell associated genes (EPCAM, SFN, KRT8, KRT18) color-coded and projected onto the UMAP embedding from A. Mono/Macro monocytes/macrophages.