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Frontiers in Pharmacology logoLink to Frontiers in Pharmacology
. 2023 Jan 18;13:1108989. doi: 10.3389/fphar.2022.1108989

Addendum: A novel N-Arylpyridone compound alleviates the inflammatory and fibrotic reaction of silicosis by inhibiting the ASK1-p38 pathway and regulating macrophage polarization

Mingming Fan 1,2, Huijuan Xiao 2,3, Dingyun Song 2, Lili Zhu 2, Jie Zhang 1, Xinran Zhang 2,4, Jing Wang 5,*, Huaping Dai 2,*, Chen Wang 1,2,5,*
PMCID: PMC9890165  PMID: 36744211

Missing information

Add information about chemical formula, Table 2, polarization and antibody list.

TABLE 2.

(Antibody list) Primary antibodies for Western Blot.

Antibodies Cat No. Manufacturer Sources of species
Fibronectin ab2413 abcam Rabbit
Collagen-Ⅰ 66761-1-Ig proteintech Mouse
IκBα #4814 cell signaling technology Mouse
p38 #8690 cell signaling technology Rabbit
P-p38 #4511 cell signaling technology Rabbit
Arginase-1 ab233548 abcam Rabbit
ASK-1 67072-1-Ig proteintech Rabbit
P-ASK-1 28846-1-AP proteintech Rabbit
NF-kB ab32536 abcam Rabbit
P-NF-kB ab76302 abcam Rabbit
iNOS 18985-1-AP proteintech Rabbit
β-Actin ab8226 abcam Mouse

Chemical formula

Chemical structure of AKEX0011

Molecular formula C18H14N5O2F3, mass 389.3 g/mol, CAS:1590403-33-0.Inline graphic

Polarization

RAW 264.7 cells are a macrophage-like, Abelson leukemia virus-transformed cell line derived from BALB/c mice. This macrophage cell line is commonly used to study phagocytosis, apoptosis, inflammation, as well as M1/M2 polarization. Polarization of M1/M2 phenotype can be determined by the expression of specific M1 (CD80, CD86) and M2 (CD206, CD163) markers detected by flow cytometry. In the in vitro experiments of our study, we detected the expression of CD86 and CD163 in RAW264.7 cells of each group by flow cytometry to explore macrophage polarization. Our flow cytometry results confirmed that silica induced polarization of RAW264.7 from M0 macrophages toward certain M1 subtype (F4/80 + CD86+), but have no effect on the polarization towards M2 subtype (F4/80 + CD163+).

Publisher’s note

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.


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