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. Author manuscript; available in PMC: 2024 Feb 4.
Published before final editing as: Gastroenterology. 2022 Aug 4:S0016-5085(22)00829-0. doi: 10.1053/j.gastro.2022.07.053

Fig 2.

Fig 2.

Intestinal bacteria modulate the level of colonic RvD1 (A) Schematic overview of experimental setup to assess the effect of gut bacteria in mice. (B) ELISA data show the levels of RvD1 in colonic tissue from wild-type mice (WT), germ-free mice (GF), GF mice conventionalized with fecal microbiota from WT mice (GF+WTm), GF mice conventionalized with Proteus mirablis (GF+PMm) or Lactobacillus rhamnosus GG bacteria (GF+LGGm). Each dot represents an individual mouse. *P<0.05, ** P<0.01 from WT (C) VMR to colorectal distention in mice shows that intracolonic administration of HC-FS (n=6) or IBS-FS (n=6) did not induce visceral hypersensitivity in germ-free mice (GF+HC-FS or GF+IBS-FS). Intracolonic administration of IBS-FS supernatant to germ-free mice that were conventionalized with fecal microbiota from wild-type mice (GF-WTm+IBS-FS, n=7) or PM bacteria (GF-PMm+IBS-FS, n=6) produced visceral hypersensitivity to mechanical distention, but not in germ-free mice conventionalized with LGG bacteria (GF-LGGm+IBS-FS, n=9). Results are expressed as mean ± SEM, *, P<0.05 from GF+IBS-FS. (D). Representative recordings of VMR to colorectal distention in GF mice conventionalized with fecal microbiota from HC individuals (HCm) or IBS-D patients (IBSm). (E) Summary graph of VMR to colorectal distention in germ-free mice conventionalized with fecal microbiota from healthy individuals (HCm, n=8) or IBS-D patients (IBSm, n=8). Results are expressed as mean ± SEM, ***, P<0.001 from GF-HCm. (F) ELISA data showing the level of RvD1 in colonic tissue from GF mice conventionalized with fecal microbiota from HC or IBS-D patient. Results are expressed as mean ± SEM, *, P<0.05. (G) qPCR data of Lox5 gene expression showing conventionalization of GF mice with the fecal material from healthy subjects (GF+HCm, n=4) or IBS-D patients (GF+IBSm, n=4) inhibited the level of Lox5 mRNA in the colonic tissues when compared to germ-free mice (n=4). **P<0.01 or ***, P<0.001 from GF. Results are expressed as mean ± SEM, ANOVA with Bonferoni post-hock test. (H) qPCR data showing the levels of mRNA for IL1b. IL6, TNFα in germ-free, germ-free mice conventionalized with microbiota from HC subjects (GF+HCm) or IBS-D patients (GF+IBSm). ***, P<0.001. ANOVA with Bonferoni post-hock test.