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[Preprint]. 2023 Jan 31:2023.01.26.525763. [Version 2] doi: 10.1101/2023.01.26.525763

Figure 5. Conformation of MBP W340A in mammalian cells by DEER.

Figure 5.

(A) Structural model and schematic of the MBP construct used for in-cell DEER. Residues substituted with Tet4-Ph are indicated in blue. The maltose-binding pocket with Trp-340 highlighted is shown as zoomed inset. (B) Distance distributions obtained by DEER for MBP211/295–Tet4-Ph-tE5 in vitro (top) and for MBP(340A)211/295–Tet4-Ph-tE5 in HEK293T cells (bottom) with uncertainties represented as shaded error bands. Dashed lines centered at the maximum of the in vitro apo (black) and maltose-bound (red) distributions are shown for ease of comparison to the in-cell distribution.