Table 1.
Second-order reaction rate constants (k2) of tetrazine ncAAs with sTCO-OH
| Tetrazine Amino Acid | Reaction Rate (× 104 M−1s−1) † | |
|---|---|---|
| Free amino acid | GFP-incorporated (TAG site) | |
| Tet-v3.0Bu | 2.1 ± 0.06 | 7.8 ± 0.30 (N150) |
| Tet-v4.0Me | 1.1 ± 0.14 | ––– |
| Tet-v4.0Ph | 2.4 ± 0.20 | 22 ± 1.3 (N150) 20 ± 1.1 (L222) |
| Tet-v4.0Pyr | 8.6 ± 1.2 | 120 ± 7.3 (N150) |
†
Second-order rate constants (k2) were obtained from plots of measured, pseudo-first order rate constants (k′) against sTCO-OH concentration (see Figures S1 and S6).