Figure 4. subdued and white walker are not required for innocuous mechanosensation, locomotion, or class III (CIII) dendritogenesis.

(A) There is no difference in innocuous touch mechanosensation (sum Kernan touch scores, three trials for each sample) in either subdued or white walker mutants (n=10 for each condition; p=0.20; BF₁₀=0.62) or GAL4-UAS-mediated CIII-knockdown (n=10 for each condition; p=0.70; BF₁₀=0.27). (B) For each genotype, there were no within-subjects effects in innocuous touch sensation across trials. w¹¹¹⁸ (n=10; p=0.69; BF₁₀=0.27); wwk^Mi/Mi (n=10; p=0.19; BF₁₀=0.85); subdued^Mi/Mi (n=10; p=0.42; BF₁₀=0.42); GAL4 control (n=10; p=0.39; BF₁₀=0.50); wwk RNAi (n=10; p=0.37; BF₁₀=0.47); subdued RNAi (n=10; p=0.80; BF₁₀=0.25). (C–G) There are no differences in acceleration (p=0.59; BF₁₀=0.13), accumulated distance (p=0.056; BF₁₀=0.96), bending behavior (p=0.71; BF₁₀=0.025), locomotion duration (p=0.22; BF₁₀=0.30), or average velocity (p=0.69; BF₁₀=0.12), between control and knockdown (n_control = 36, n_wwk = 35, and n_subdued = 36). (H–J) Representative neural images of CIII ddaF neuron dendritic arbors under control and knockdown conditions. (K–M) There is no difference in total dendritic length (p=0.48; BF₁₀=0.34), number of branches (p=0.70; BF₁₀=0.27), or dendritic branch density (p=0.81; BF₁₀=0.24) between control and knockdown (n=10 for each condition).