Figure 4. IDRs of partitioned factors are sufficient and necessary to recapitulate selective partitioning.

A) MED1^IDR partitioned proteins are themselves enriched for IDRs.

B) PONDR plots (VSL2). Black bar highlights IDR purified as an mCherry fusion.

C) Proteomics data from MED^IDR pelleting experiments showing replicate log₂(pellet/sup) values for each protein.

D) Cartoon depiction of purified mCherry-IDRs tested. See also Figure S4A.

E) Images of indicated mCherry-IDR partitioning into mEGFP-MED1^IDR droplets in total RNA. Scale, 5μm. See also Figure S4B.

F) Boxplot(10–90%) of condensed fraction. p-values, one-way ANOVA vs no IDR control.

G) Cartoon depiction of experimental setup.

H) Images of mCherry-IDRs (top row) tested for partitioning into CFP-LacI-MED1^IDR foci (bottom row). Scale, 5μm.

I) Boxplot(min-max) of partition coefficients for experiments in 4H. p-values, one-way ANOVA vs no IDR control.

J) Diagram of CTR9 and SPT6 truncations as mCherry fusions tested for partitioning into CFP-LacI-MED1^IDR in Lac array cells.

K) Same as 4H, but for indicated mCherry fusions.

L) Same as 4I, but for indicated mCherry fusions.

See also Figure S4.