Figure 8: Total reactive and neutralising titres against virus.
In addition, the sera were also used to assess reactivity with virus samples (identical serum samples as used above). (A) Serum samples were tested for reactivity with virus by ELISA. To each well, approximately 200 AgU of virus was added, and sera were assessed at dilutions between 1:250 and 1:8000 in duplicate. Samples were considered positive if the average OD was >2x that of the negative (no serum) control wells within each plate. Graph shows the endpoint titre for individual animals, mean ± SEM, n = 8 in duplicate (n = 7 for PBS adj+ group). (B) Virus neutralisation was assessed against 100 TCID50 of genogroup B2 EVA71, neutralising titres were determined using the Spearman-Karber method. Graph shows mean titres from individual animals, as well as group mean ± SEM, n = 8. Abbreviations: adj−; unadjuvanted immunogen, adj+; adjuvanted immunogen, virus; genotype B2 virus immunogen, 18/116; genogroup C4 virus immunogen.