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[Preprint]. 2023 Feb 2:2023.02.01.23285349. [Version 1] doi: 10.1101/2023.02.01.23285349

Figure 1. Detection of vaccine-derived Spike protein mRNA in human placental explants and four human cell lines using qRT-PCR.

Figure 1.

(A) Vaccine messenger RNA (mRNA) standard curves. Standard curves for mRNA-1273 and BNT162b2 vaccines were generated to calculate vaccine-derived Spike mRNA concentrations in placental explants and cells. The limit of detection (LOD) level was set according to the standard curve (0.05 pg/μL, mRNA-1237 CT = 23.2, BNT162b2 CT = 24.6). (B) Vaccine mRNA detected in placental explants (villi) and four different types of cell lines exposed to mRNA-1273 or BNT162B2 at 0.1 μg/mL or 1 μg/mL for 0.5h or 4h. Results were calculated by extrapolating CT values based on the derived standard curves for each mRNA vaccine. Black dotted line represents the LOD level of 0.05 pg/μL. Grey box represents the area under LOD. All data represent 2 independent experiments. CT = Cycle threshold.