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[Preprint]. 2023 Feb 2:2023.02.01.23285349. [Version 1] doi: 10.1101/2023.02.01.23285349

Figure 3. Expression of inflammatory mediators in conditioned media of placental explants incubated with COVID mRNA vaccine.

Figure 3.

Chorionic villi explants derived from 2nd or 3rd trimester human placenta (n=6) were incubated with 0.1 μg/mL or 1 μg/mL mRNA-1237 (n=4) or BNT162B2 (n=2) vaccines for 0.5h or 4h. Conditioned media was collected and levels of 20 unique cytokines were measured using the Luminex multiplex assay. (A) Distribution in abundance of cytokines in all samples. Black bars represent median levels in cytokine abundance. (B) Hierarchical clustering of samples by levels of cytokines. We excluded cytokines of low variance from this analysis (n=6 cytokines). In general, sample profiles clustered by culture time (0.5 vs. 4h). (C) Significance of factors in cytokine expression evaluated using multiple linear regression models. Asterisks represent significance cutoffs: p<0.05 (*); p<0.005 (**); and p<0.0005 (***). Abbreviations listed for factors assessed in our analysis: mRNA-1237 (M), BNT162B2 (P) vaccines, culture time (Time), or trimester (Age).