Genetics Correction for “Leveraging a natural murine meiotic drive to suppress invasive populations,” by Luke Gierus, Aysegul Birand, Mark D. Bunting, Gelshan I. Godahewa, Sandra G. Piltz, Kevin P. Oh, Antoinette J. Piaggio, David W. Threadgill, John Godwin, Owain Edwards, Phillip Cassey, Joshua V. Ross, Thomas A. A. Prowse and Paul Q. Thomas, which published November 8, 2022; 10.1073/pnas.2213308119 (Proc. Natl. Acad. Sci. U.S.A. 119, e2213308119).
The authors note: “We inadvertently used the term ‘Gender’ as a table heading in Fig. 1C. We would like to substitute ‘Gender’ with ‘Sex’ as this is the correct term. Sex is biologically defined as male or female. This is the correct term for Fig. 1C and the term we should have used. In contrast, gender is a social construct and is not necessarily binary. Gender is not the correct term for this table and we are keen to avoid any unnecessary confusion or unintended offense.”
Fig. 1.
Overview of t haplotype modification strategies for population suppression. (A) The integration of a transgene within the t haplotype expressing Cas9 under the control of a male-specific promoter or a germline-specific promoter, coupled with a ubiquitously expressed gRNA targeting a haplosufficient female fertility gene. (B) Inheritance of tw2 is biased in males but not females. In the tCRISPR system, Cas9 is only active in males and, with a ubiquitously expressed gRNA, disrupts a haplosufficient female fertility gene in the germline. tCRISPR males transmit the tCRISPR transgene and a disrupted fertility gene to ∼95% and 100% of offspring, respectively. The tCRISPR(2) strategy is identical except that the Cas9 is active in the male and female germline, thereby generating a more rapid increase in female infertility alleles. Prl* mice contain a sequence difference at the gRNA target site but maintain a functional protein, making these mice resistant against any further cleavage at that site. (C) Fertility of male and female mice carrying the various versions of chromosome 17 and Prl within the target chromosome.
The online version has been corrected.