Appendix 1—table 2. List of antibodies and other reagents used for flow cytometry analyses.
| Target | Clone | Conjugate | Source | Catalog number |
|---|---|---|---|---|
| CD3 | 17A2 | APC/Fire-810 | BioLegend | 100267 |
| CD4 | RM4-5 | APC | eBioscience | 17-0042-83 |
| CD8 | RPA-T8 | PE-Cy5 | BD Biosciences | 561951 |
| CD9 | MZ3 | PerCP-Cy5.5 | BioLegend | 124817 |
| CD11b | M1/70 | PE-Cy7 | eBioscience | 25-0112-82 |
| CD11c | HL3 | V450 | BD Biosciences | 560521 |
| CD19 | 1D3 | BV480 | BD Biosciences | 566107 |
| CD36 | HM36 | PE | BioLegend | 102606 |
| CD45 | 30-F11 | BV785 | BioLegend | 103149 |
| CD64 | X54-5/7.1 | PE-DAZZLE594 | BioLegend | 139320 |
| CD90.2 | 30-H12 | Alexa Fluor 700 | BioLegend | 105319 |
| CLEC2 | 17D9 | FITC | Bio-Rad | MCA5700 |
| F4/80 | BM8 | BV711 | BioLegend | 123147 |
| Ly6C | HK1.4 | APC-Cy7 | BioLegend | 128025 |
| Ly6G | 1A8 | BV650 | BioLegend | 127641 |
| MHC-II | M5/114.15.2 | BV750 | BD Biosciences | 747458 |
| MHC-II | M5/114.15.2 | Alexa Fluor 700 | Thermo Fisher | 56-5321-82 |
| NK1.1 | PK136 | PerCP-Cy5.5 | BioLegend | 108727 |
| Siglec-F | E50-2440 | PE | BD Biosciences | 552126 |
| Siglec-F | E50-2440 | BV605 | BD Biosciences | 740388 |
| TIM4 | 54 (RMT4-54) | PerCP-eFluor710 | Thermo Fisher | 46-5866-82 |
| XCR1 | ZET | BV421 | BioLegend | 148216 |
| Other reagents | ||||
| Zombie NIR Fixable Viability Kit | BioLegend | 423106 | ||
| True-Stain Monocyte Blocker | BioLegend | 426103 | ||
| Brilliant Stain Buffer Plus | BD Biosciences | 566385 | ||