Table 3.
The sequences of primers used in generating deletion mutants and the lppA complementation strain.
| Primers | Sequences (5’-3’) |
|---|---|
| P1a (lppA-up-F) | CGCGGATCCACCGCTTGTGCAGGTGTCACTGCTCGAACA |
| P2a (lppA-up-R) | TTTATCTTGTGCAATGACCACCACCACAAGCTACTAAGAT |
| P3a (lppA-down-F) | CAGAATTGGTTAATTGGGGGCAACTGCTACCAAAGAC |
| P4a (lppA-down-R) | TAACTGCAGACAAGCGGTTGCCTCTGGAGGTGAGCTATCAC |
| P1b (lolB-up-F) | CGCGGATCCACCGCTTGTATCGACACGAGTTGAAAG |
| P2b (lolB-up-R) | TTTATCTTGTGCAATGGTCTAGGTACTAGTCACTC |
| P3b (lolB-down-F) | CAGAATTGGTTAATTGGCGAGATTATCGCCAATT |
| P4b (lolB-down-R) | TAACTGCAGACAAGCGGTCGATTGGCTTCATTGCTT |
| P1c (metQ-up-F) | CGCGGATCCACCGCTTGTCGTATCGGTAGTGCAACT |
| P2c (metQ-up-R) | TTTATCTTGTGCAATGGTGTTCAAAGGTGCGATG |
| P3c (metQ-down-F) | CAGAATTGGTTAATTGCCACGCCATCTTTGAAGT |
| P4c (metQ-down-R) | TAACTGCAGACAAGCGGTCGCTGAGTTACAAGATGG |
| P1d (plpA-up-F) | CGCGGATCCACCGCTTGTGACTTGCCATACAGTGCA |
| P2d (plpA-up-R) | TTTATCTTGTGCAATGCAGAGTAAGGGATAGCAG |
| P3d (plpA-down-F) | CAGAATTGGTTAATTGTGGGCGTACTAATATGGC |
| P4d (plpA-down-R) | TAACTGCAGACAAGCGGTTACCACTTCTCTTTCGGC |
| P5 (Kan-F) | CATTGCACAAGATAAAAATATAT |
| P6 (Kan-R) | CAATTAACCAATTCTGATTAG |
| P7 (C-lppA-F) | CGGGGTACCAGTTAAGGGCAGCTTAAGTTA |
| P8 (C-lppA-R) | TATGAGCTCTTTGTCTTTGGTAGCAGTTGC |
| P9 (lppA-test-F) | TATTTGGCAGGGCTTCTCGA |
| P10 (lppA-test-F) | AATTGCCCTGCGTGTCTTTT |