REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial Strains
Mab sensu stricto, strain CIP104536T, smooth	Laboratoire de Référence des Mycobactéries (IP, France)	ATCC19977T
ΔahpCe/ahpCi-ha: Unmarked deletion of ahpC in Mab S expressing an ATc-regulatory copy of ahpC	This study	N/A
E. coli XL1-Blue	Stratagene	Cat#200249
Oligonucleotides
Primers are listed in Table S1 Merck N/A
Critical Commercial Assays
Hygromycin B	Sigma-Aldrich	Cat#H3274
Kanamycin	Euromedex	Cat#EU0420-B
Ampicillin	Euromedex	Cat#EU0400-E
Difco Middlebrook 7H9 Broth	Thermo Fisher Scientific	Cat#DF0713-17-9
Difco Middlebrook 7H10 Broth	Thermo Fisher Scientific	Cat#DF0627-17-4
Middlebrook OADC Growth Supplement	Sigma-Aldrich	Cat#M0678
Tween-80	Sigma-Aldrich	Cat#P1754
Q5 DNA Polymerase	New England Biolabs	Cat#M0491L
T4 DNA Ligase	New England Biolabs	Cat#M0202S
NucleoSpin Plasmid Kit	Machery-Nagel	Cat#740588.50
NucleoSpin Gel and PCR Clean-up	Machery-Nagel	Cat#740609.50
GenElute HP Plasmid Midiprep Kit	Sigma-Aldrich	Cat#NA0200-1KT
Pierce Silver Stain Kit	Life-Technologies	Cat#24612
DAPI Solution	Becton Dickinson	Cat#564907
In-Fusion	TAKARA	Cat#638947
Anhydrotetracycline	Merck	Cat#37919-100MG-R
Isoniazid	Merck	Cat#I3377-250G
BCA assay	Thermo Fisher Scientific	Cat#23227
BSA	New England Biolabs	Cat#B9000S
SuperSignal West Femto Maximum Sens	Life-Technologies	Cat#34095
Pierce BCA Protein Assay Kit	Thermo Scientific	Cat#23227
Recombinant DNA
pTEC27	Takaki K et al.64	Lalita Ramakrishnan, Cat#30182
pUX1-katG	Daher W et al.8	N/A
pGEX-2T	Silvie O et al.28	N/A
pET30	Sigma-Aldrich	Cat#69077-3
pVV16	Vilchèze C et al.65	N/A
pMV306	Stover C.K et al.66	N/A
pMV261	Stover C.K et al.66	N/A
Software and Algorithms
Prism 9.0	Graphpad	https://www.graphpad.com
Zen (Blue edition)	Zeiss	https://www.zeiss.com/microscopy/int/products/microscope-software/zen.htmL
Experimental Models: Cell Lines
THP-1 macrophages	This paper	ATCC® TIB-202™
A549	This paper	ATCC® CCL-185™
A549-CD81-LEL-KO	This paper	N/A
Primary human monocyte-derived macrophages	Etablissement Français du sang (EFS), France	N/A
Antibodies
Rat anti-HA (3F10)	Merck	Cat#11867423001; RRID:AB_390918
Rat anti-KasA	Viljoen A et al.67	N/A
Mouse anti-His	Merck	Cat#H1029-.2ML; RRID:AB_260015
Rabbit anti-BSA	Fisher Scientific	Cat#10092782
Rabbit anti-CD81	Abcam	Cat#ab109201; RRID:AB_10866464
Rabbit anti-Actin	Abcam	Cat#ab179467; RRID:AB_2737344
Mouse anti-GFP	Antibodies-online	Cat#ABIN387748
Mouse anti-HU CD43	Becton Dickinson	Cat#555474; RRID:AB_2638919
Mouse anti-HU CD82	Antibodies-online	Cat#ABIN1383874
Mouse anti-HU CD151	Becton Dickinson	Cat#556056; RRID:AB_2646510
Mouse anti-HU CD206/MR	Becton Dickinson	Cat#555953; RRID:AB_2649408
Mouse anti-HU CR3/CD11B	Life Technologies	Cat#4-0118-82
Mouse anti-HU CD81	Becton Dickinson	Cat#555675; RRID:AB_396028
Mouse anti-HU CD9	Becton Dickinson	Cat#555370; RRID:AB_395772
Mouse anti-HU CD63	Becton Dickinson	Cat#556019; RRID:AB_2646310
Mouse IgG1	Becton Dickinson	Cat#555746; RRID:AB_2648489
Alexa Fluor 488 anti-Mouse IgG (Goat)	Life Technologies	Cat#A11017; RRID:AB_2758366
Alexa Fluor 594 anti-Mouse IgG (Goat)	Life Technologies	Cat#A11032; RRID:AB_2534091
Anti-Rabbit IgG H&L (HRP) (Goat)	Abcam	Cat#ab205718; RRID:AB_2819160
Anti-mouse IgG H&L (HRP) (Goat)	Abcam	Cat#ab205719; RRID:AB_2755049
Anti-Rat IgG H&L (HRP) (Goat)	Abcam	Cat#ab97057; RRID:AB_10680316
Alexa Fluor 647 anti-Mouse IgG (Donkey)	ThermoFisher Scientific	Cat#A32787; RRID:AB_2762830
Chemicals, Peptides, and Recombinant Proteins
Hydrogen peroxide	Merck	Cat#107209
Anhydride acetic acid	Merck	Cat#691275
Acetonitrile	Merck	Cat#34851
Trifluoroacetic acid	Merck	Cat#302031
Other
Glutathione Sepharose 4FF, 25 ml	Sigma	Cat#GE17-5132-01
NI Sepharose 6FF, 25 ml	Sigma	Cat#GE17-5318-01
HA-Tag IP/Co-IP	Thermo Fisher Scientific	Cat#26180
GFP-Trap Agarose	Chromotek	Cat#gta-20
Latex beads, carboxylated, fluorescent yellow-green	Sigma	Cat#L4655-1ML
Phosphate Buffer Saline	Euromedex	Cat#ET330
Bovine Serum Albumin	Sigma	Cat#A0281