表 3. Amplification linear ranges and standard curves of real- time PCR for cytokines and GAPDH of tree shrew .
树鼩细胞因子及 GAPDH 荧光定量 PCR 扩增线性范围 及标准曲线
| 项目种类 | 线性范围
(copies/5 μL) |
标准曲线 | ||
| 斜率 | 截距 | 线性相关
系数 R2 |
||
| IL-6 | 1 × 1010~10 | −3.555 | 41.150 | 0.993 |
| IL-8 | 1 × 1010~1 × 103 | −3.793 | 47.371 | 0.993 |
| IL-10 | 1 × 1010~100 | −3.530 | 43.148 | 0.997 |
| IL-17A | 1 × 1010~10 | −3.366 | 40.325 | 0.996 |
| IFN-γ | 1 × 1010~1 × 103 | −3.628 | 41.740 | 0.995 |
| GAPDH | 1 × 1010~100 | −3.535 | 44.260 | 0.995 |