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. 2019 Jun;36(3):407–413. [Article in Chinese] doi: 10.7507/1001-5515.201801036

表 3. Amplification linear ranges and standard curves of real- time PCR for cytokines and GAPDH of tree shrew .

树鼩细胞因子及 GAPDH 荧光定量 PCR 扩增线性范围 及标准曲线

项目种类 线性范围
(copies/5 μL)
标准曲线
斜率 截距 线性相关
系数 R2
IL-6 1 × 1010~10 −3.555 41.150 0.993
IL-8 1 × 1010~1 × 103 −3.793 47.371 0.993
IL-10 1 × 1010~100 −3.530 43.148 0.997
IL-17A 1 × 1010~10 −3.366 40.325 0.996
IFN-γ 1 × 1010~1 × 103 −3.628 41.740 0.995
GAPDH 1 × 1010~100 −3.535 44.260 0.995