| Antibody |
Anti-mouse CD45 APC/Cyanine7 (Rat monoclonal) |
BioLegend |
cat.# 103115 |
1:200 |
| Antibody |
Anti-mouse/human CD45R/B220 Pacific Blue (Rat monoclonal) |
BioLegend |
cat.# 103230 |
1:200 |
| Antibody |
Anti-mouse F4/80 APC (Rat monoclonal) |
BioLegend |
cat.# 123115 |
1:100 |
| Antibody |
Anti-mouse F4/80 FITC (Rat monoclonal) |
BioLegend |
cat.# 123107 |
1:100 |
| Antibody |
Anti-mouse/human CD11b PE/Cyanine7 (Rat monoclonal) |
BioLegend |
cat.# 101215 |
1:200 |
| Antibody |
Anti-mouse Treml4 PE (Rat monoclonal) |
BioLegend |
cat.# 143303 |
1:100 |
| Antibody |
Anti-mouse TER-119 Pacifin Blue (Rat monoclonal) |
BioLegend |
cat.# 116231 |
1:300 |
| Antibody |
Anti-mouse TER-119 FITC (Rat monoclonal) |
BioLegend |
cat.# 116205 |
1:300 |
| Antibody |
Anti-mouse CD71 APC (Rat monoclonal) |
BioLegend |
cat.# 113819 |
1:100 |
| Antibody |
Anti-mouse CD71 PE (Rat monoclonal) |
BioLegend |
cat.# 113807 |
1:100 |
| Antibody |
Anti-mouse TER-119 PE (Rat monoclonal) |
BioLegend |
cat.# 116207 |
1:300 |
| Antibody |
Anti-mouse CD45 PE/Cyanine7 (Rat monoclonal) |
BioLegend |
cat.# 147703 |
1:200 |
| Antibody |
Anti-mouse FPN (Rat monoclonal) |
Amgen |
DOI:10.1172/JCI127341
|
1:100 |
| Antibody |
Anti-mouse Gr-1 Pacific Blue (Rat monoclonal) |
BioLegend |
cat.# 108429 |
1:200 |
| Antibody |
Anti-mouse MHCII Pacific Blue (Rat monoclonal) |
BioLegend |
cat.# 107619 |
1:200 |
| Antibody |
Anti-human/mouse Ferritin Heavy Chain (FTH1) (Rabbit polyclonal) |
Cell Signaling Technology |
cat.# 3998 |
1:100 |
| Antibody |
Anti-mouse AXL APC (Rat monoclonal) |
Invitrogen |
cat.# 17-1084-82 |
1:100 |
| Antibody |
Anti-mouse MERTK FITC (Rat monoclonal) |
BioLegend |
cat.# 151503 |
1:100 |
| Antibody |
Anti-mouse Tim-4 Alexa Fluor 647 (Rat monoclonal) |
BioLegend |
cat.# 130007 |
1:100 |
| Antibody |
Anti-mouse Stab2 Alexa Fluor 488 (Rabbit polyclonal) |
G-Biosciences |
cat.# ITN2255 |
1:100 |
| Antibody |
Anti-mouse/human Ferritin Light Chain (FTL) (Rabbit polyclonal) |
Abcam |
cat.# ab69090 |
1:100 |
| Antibody |
Anti-mouse HO-1 (Rabbit polyclonal) |
Enzo Life Sciences |
cat.# ADI-OSA-150 |
1:400 |
| Antibody |
Anti-mouse Ki-67 Alexa Fluor 488 (Rat monoclonal) |
BioLegend |
cat.# 652417 |
1:100 |
| Antibody |
Anti-Rabbit IgG (H+L) Alexa Fluor 488 (Donkey polyclonal) |
Thermo Fisher Scientific |
cat.# A-21206 |
1:200 |
| Antibody |
Anti-Rabbit IgG (H+L) Alexa Fluor 647 (Donkey polyclonal) |
ThermoFisher Scientific |
cat.# A-31573 |
1:200 |
| Antibody |
Anti-mouse CD16/32 TruStain FcX (Rat monoclonal) |
BioLegend |
cat.# 101319 |
1:100 |
| Antibody |
Anti-mouse Gr-1 Biotin (Rat monoclonal) |
BioLegend |
cat.# 108403 |
1:100 |
| Antibody |
Anti-mouse/human CD45R/B220 (Rat monoclonal) |
BioLegend |
cat.# 103204 |
1:100 |
| Antibody |
Anti-mouse CD3 (Rat monoclonal) |
BioLegend |
cat.# 100243 |
1:100 |
| Antibody |
Anti-mouse Ly-6C (Rat monoclonal) |
BioLegend |
cat.# 128003 |
1:100 |
| Chemical compound, drug |
N-Acetyl-L-cysteine (NAC) |
Sigma-Aldrich |
cat.# A7250 |
2 g/L in drinking water |
| Chemical compound, drug |
Iron-Dextran Solution |
Sigma-Aldrich |
cat.# D8517 |
8 mg |
| Chemical compound, drug |
Hemin |
Sigma-Aldrich |
cat.# 51280 |
20 µM |
| Chemical compound, drug |
Ferric Ammonium Citrate (FAC) |
Sigma-Aldrich |
cat.# F5879 |
150, 50 and 10 µM |
| chemical compound, drug |
CORM-A1 |
Sigma-Aldrich |
cat.# SML0315 |
50 µM |
| Chemical compound, drug |
Deferoxamine (DFO) |
Sigma-Aldrich |
cat.# D9533 |
100 µM |
| Chemical compound, drug |
Zinc (II) Protoporphyrin IX (ZnPP) |
Sigma-Aldrich |
cat.# 691550 |
5, 1 and 0.5 µM |
| Chemical compound, drug |
Tunicamycin (Tm) |
Sigma-Aldrich |
cat.# T7765 |
2.5 µM |
| Chemical compound, drug |
Liproxstatin-1 (Lip-1) |
Sigma-Aldrich |
cat.# SML1414 |
2 µM |
| Chemical compound, drug |
EZ-Link Sulfo-NHS Biotin |
Thermo Fisher Scientific |
cat.# 21217 |
1 mg / 100 µL |
| Commercial assay, kit |
LIVE/DEAD Fixable Aqua |
Invitrogen |
cat.# L34966
|
|
| Commercial assay, kit |
LIVE/DEAD Fixable Violet |
Invitrogen |
cat.# L34964
|
|
| Commercial assay, kit |
CellROX Deep Red Reagent |
Invitrogen |
cat.# C10422
|
|
| Commercial assay, kit |
Lipid Peroxidation Assay Kit |
Abcam |
cat.# ab243377 |
|
| Commercial assay, kit |
MitoSOX Red |
Invitrogen |
cat.# M36008
|
|
| Commercial assay, kit |
Lysosomal Intracellular Activity Assay Kit |
Biovision |
cat.# K448 |
|
| Commercial assay, kit |
Me4BodipyFL-Ahx3Leu3VS (Fluorescent Proteasome Activity) |
R&D Systems |
cat.# I-190 |
|
| Commercial assay, kit |
tetramethylrhodamine ethyl ester - TMRE (Fluorescent Probe) |
Sigma-Aldrich |
cat.# 87917 |
|
| Commercial assay, kit |
MitoTracker Green |
Invitrogen |
cat.# M7514 |
|
| Commercial assay, kit |
Cal-520 AM Probe |
Abcam |
cat.# ab171868 |
|
| Commercial assay, kit |
FerroOrange |
DojinD |
cat.# F374 |
|
| Commercial assay, kit |
PROTEOSTAT Aggresome Detection Kit |
Enzo Life Sciences |
cat.# ENZ-51035–0025 |
|
| Commercial assay, kit |
ATP Fluorometric Assay Kit |
Sigma-Aldrich |
cat.# MAK190 |
|
| Commercial assay, kit |
Iron Assay Kit |
Sigma-Aldrich |
cat.# MAK025 |
|
| Commercial assay, kit |
PKH-67 |
Sigma-Aldrich |
cat.# MIDI67-1KT |
|
| Commercial assay, kit |
Heme Assay Kit |
Sigma-Aldrich |
cat.# MAK316 |
|
| Commercial assay, kit |
SFBC |
Biolabo |
cat.# 80008 |
|
| Commercial assay, kit |
UIBC |
Biolabo |
cat.# 97408 |
|
| Commercial assay, kit |
Mouse Erythropoietin/EPO Quantikine ELISA Kit |
R&D Systems |
cat.# MEP00B |
|
| Commercial assay, kit |
IL-6 Quantikine ELISA Kit |
R&D Systems |
cat.# M600B |
|
| Commercial assay, kit |
Mouse Hemopexin ELISA Kit |
Abcam |
cat.# ab157716 |
|
| Commercial assay, kit |
Direct-zol RNA Microprep Kit |
Zymo Research |
cat.# R2062 |
|
| Commercial assay, kit |
Alexa Fluor 488 Conjugation Kit (Fast) - Lightning-Link |
Abcam |
cat.# ab236553 |
|
| Commercial assay, kit |
Alexa Fluor 647 Conjugation Kit (Fast) - Lightning-Link |
Abcam |
cat.# ab269823 |
|
| Commercial assay, kit |
RevertAid H Minus Reverse Transcriptase |
Thermo Fisher Scientific |
cat.# EP0452 |
|
| Commercial assay, kit |
SG qPCR Master Mix |
EURx |
cat.# E0401 |
|
| Commercial assay, kit |
Iron Stain Kit |
Sigma-Aldrich |
cat.# HT20-1KT |
|
| Commercial assay, kit |
Iron Stain Kit |
Abcam |
cat.# ab150647 |
|
| Commercial assay, kit |
In Situ Cell Death Detextion Kit |
Roche |
cat.# 11684795910 |
|
| Other |
Standard iron content diet for mice |
SAFE |
cat.# U8958v0177 |
Diet for young and aged mice |
| Other |
Reduced iron content diet for mice |
SAFE |
cat.# U8958v0294 |
Diet for aged IR mice |
| Other |
Collagenase D |
Roche |
cat.# 11088882001 |
Digestion of spleen for sorting |
| Other |
Trizol-LS |
Invitrogen |
cat.# 10296010 |
RNA isolation reagent for RNA-seq |
| Other |
Normal Rat Serum |
Thermo Fisher Scientific |
cat.# 10,710 C |
Blocking reagent for flow cytometry |
| Other |
MojoSort Streptavidin Nanobeads |
BioLegend |
cat.# 480016 |
Beads for magnetic sorting of RPMs and aggreagtes |
| Other |
MojoSort Streptavidin Nanobeads |
BioLegend |
cat.# 480072 |
Beads for magnetic sorting of RPMs and aggreagtes |
| Other |
EasyEights EasySep Magnet |
STEMCELL |
cat.# 18103 |
Magnet used for magnetic sorting of RPMs and aggregates |
| Other |
LS Separation Column |
Milyenyi Biotec |
cat.# 130-042-401 |
Magnet used for magnetic sorting of aggregates |
| Other |
CPDA-1 |
Sigma-Aldrich |
cat.# C4431 |
Anticoagulant used for blood collection for transfusions |
| Other |
Accutase |
BioLegend |
cat.# 423201 |
Reagent used for cells detachment in vitro
|
| Other |
Zymosan A BioParticles Alexa Fluor 488 |
Invitrogen |
cat.# Z23373
|
Cargo for ex vivo phgocytosis |
| Other |
TRIzol Reagent |
Invitrogen |
cat.# 15596018 |
RNA isolation reagent |
| Peptide, recombinant protein |
IL-33 |
BioLegend |
cat.# 580506 |
10 ng/mL |
| Peptide, recombinant protein |
Mini-hepcidin (PR73) |
Gift from Elizabeta Nemeth, UCLA, USA |
https://doi.org/10.1128/IAI.00253-18
|
2 µg/mL |
| Peptide, recombinant protein |
Macrophage Colony-Stimulating Factor (M-CSF) |
BioLegend |
cat.# 576406 |
20 ng/mL |
| Sequence-based reagent |
HAMP Forward |
This paper |
Real-time PCR primers |
5’ ATACCAATGCAGAAGAGAAGG-3’ |
| Sequence-based reagent |
HAMP Reverse |
This paper |
Real-time PCR primers |
5’-AACAGATACCACACTGGGAA-3’ |
| Software, algorithm |
FlowJo |
FlowJo |
v10.8.1 |
|
| Software, algorithm |
CytExpert |
Beckman Coulter |
v2.4 |
|
| Software, algorithm |
GraphPad Prism |
GraphPad Software |
v9 |
|
