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. 2023 Feb 13;11:e14826. doi: 10.7717/peerj.14826

Figure 5. Bre decreased HG-induced podocyte injury by inhibiting NLRP3-mediated pyroptosis in vitro.

Figure 5

(A) After being treated with HG, MCC950 (10 μM), and Ac-YVAD-cmk (40 μM) for 48 h, MPC5 cell viability was assessed using CCK-8 assays. (B) After being treated with HG, MCC950 (10 μM), and Ac-YVAD-cmk (40 μM) for 48 h, the mRNA levels of α-SMA and synaptopodin in MPC5 cells were assessed using qRT‐PCR. qRT‐PCR (C), western blotting (D and E), and IF (F) analysis were carried out to assess α-SMA and synaptopodin levels in MPC5 cells after treatment with nigericin (10 μM) for 48 h in the presence of HG and Bre. **p < 0.01.