Figure 1. Ferroptosis is more prevalent in cardiomyocyte than apoptosis and necroptosis in infarcted postnatal hearts.
LAD-O was performed at either P1 or P7, heart tissue section prepared at 1, 3 or 6 DPMI. (A-G) Infarct zone stained for cCasp3 (Magenta), MF20 (green) and DAPI (blue). Arrow, cardiomyocytes positive for cCasp3, ratio quantified in (G). Arrowhead, non-cardiomyocyte positive for cCasp3. (H-N) Infarct zone stained for pMLKL (Magenta), cTnT (green) and DAPI (blue). Arrow, cardiomyocytes positive for pMLKL, ratio quantified in (N). Arrowhead, non-cardiomyocyte positive for pMLKL. (O-U) Infarct zone stained for Ptgs2 (Magenta), cTnT (green) and DAPI (blue). Arrow, cardiomyocytes positive for Ptgs2, ratio quantified in (U). Error bars indicate SD. *, p<0.05. NS, not significant. NS in A, no significance among all groups. #, infarct zone. Scale bar, 25 μm (A-F, H-M, O-T).