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. 2023 Feb 16;14:879. doi: 10.1038/s41467-023-36632-8

Author Correction: SpG and SpRY variants expand the CRISPR toolbox for genome editing in zebrafish

Fang Liang 1,2, Yu Zhang 1, Lin Li 1, Yexin Yang 2, Ji-Feng Fei 3, Yanmei Liu 1,, Wei Qin 1,
PMCID: PMC9935635  PMID: 36797310

Correction to: Nature Communications 10.1038/s41467-022-31034-8, published online 14 June 2022

The original version of this Article contained an error in the Methods section “Cas9 RNP complex preparation, microinjection and image acquisition in zebrafish”, which incorrectly read ‘One-cell stage zebrafish embryos were injected with 2 nl of a solution containing 5 µM RNP complex or 300 ng/μl Cas9 mRNA and 30 ng/μl gRNA mixture. For SpRY-CBE4max or zSpRY-ABE8e mRNA, 400 ng/μl was used. For the HDR experiment, 300 ng/μl Cas9 mRNA, 30 ng/μl gRNA and 20 ng/μl donor templated were mixed and injected.’

The correct version states ‘One-cell stage zebrafish embryos were injected with 2 nl of a solution containing 5 µM RNP complex or 300 ng/μl Cas9 mRNA and 200 ng/μl gRNA mixture. For SpRY-CBE4max or zSpRY-ABE8e mRNA, 400 ng/μl was used. For the HDR experiment, 300 ng/μl Cas9 mRNA, 200 ng/μl gRNA and 20 ng/μl donor templated were mixed and injected.’

This has been corrected in both the PDF and HTML versions of the Article.

Contributor Information

Yanmei Liu, Email: yanmeiliu@m.scnu.edu.cn.

Wei Qin, Email: qinwei@scnu.edu.cn.


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