Fig. 1.

Improved cell yields and final OPC yields with optimized papain digestion. A. Quantification of total cell yields (expressed as x10⁶/mouse brain) following papain digestion in the Emery & Dugas (2013) protocol with non-humidified 95% O₂/5% CO₂ gas[8], without gas (95% O₂/5% CO₂) (n = 5) and with humidified 95% O₂/5% CO₂ gas (n = 35). B. Quantification of OPC yields (expressed as x10⁶/mouse brain) following papain digestion in the Emery & Dugas (2013) protocol with non-humidified 95% O₂/5% CO₂ gas[8], without gas (95% O₂/5% CO₂) (n = 5) and with humidified 95% O₂/5% CO₂ gas (n = 35). Single sample t-test. Data represented as mean $\pm$ SEM. C. Image of the petri lid used for perfusion. D. Schematic of the 95% O₂/5% CO₂ gas perfusion set-up. E. Image of the 95% O₂/5% CO₂ gas perfusion set-up.