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. 2023 Feb 2;10:102051. doi: 10.1016/j.mex.2023.102051

Fig. 3.

Fig 3

Immunocytochemistry characterization of cultures and purification from Olig2-Cre YFP+ mice. A. OPCs at 7 DIV under proliferative conditions positively labelled for PDGFRα, NG2 and Olig2, B. Immature oligodendrocytes after 2 DIV in differentiation/T3-containing media stained for O4. C. Oligodendrocytes after 3 DIV in differentiation/T3-containing media stained for MOG and Sox10 or D. MBP. E. Olig2-Cre YFP+ transgenic mouse. F. OPCs from Olig2-Cre YFP+ transgenic mice in culture (2 DIV, EVOS light microscope, YFP light cube) Antibodies: rat anti-PDGFRα (BD Pharmigen, 1:100), rabbit anti-NG2 (Milipore, 1:200), goat anti-Olig2 (R&D, 1:200), mouse anti-O4 (R&D, 1:200), goat anti-Sox10 (R&D, 1:250) rat anti-MOG (R&D, 1:300), rat anti-MBP (Novus, 1:500), AlexaFluor 488 donkey anti-rat (ThermoFisher, 1:1000), AlexaFluor 647 donkey anti-rabbit (Jackson ImmunoResearch, 1:500), AlexaFluor 594 anti-mouse (Jackson ImmunoResearch, 1:500), AlexaFluor 555 donkey anti-goat (Invitrogen, 1:500). Nuclei stained with DAPI. Labeling imaged using Zeiss LSM780 confocal microscope, YFP+ cells imaged with an EVOS light microscope. Scale bar, 20 µm.