REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
pan-Keratin	Cell Signaling Technology	Cat# 4545; RRID:AB_490860
Emerin	Cell Signaling Technology	Cat# 5430; RRID:AB_10691714
ERp57	Cell Signaling Technology	Cat# 2881; RRID:AB_2160840
B23-NPM	Cell Signaling Technology	Cat# 3542; RRID:AB_2155178
Nup98	Cell Signaling Technology	Cat# 2598; RRID:AB_2267700
β-actin	Cell Signaling Technology	Cat# 3700; RRID:AB_2242334
eEF2k	Cell Signaling Technology	Cat# 3691; RRID:AB_2097313
eIF4E	Cell Signaling Technology	Cat# 9742; RRID:AB_823488
4E-BP1	Cell Signaling Technology	Cat# 9644; RRID:AB_2097841
eIF4B	Cell Signaling Technology	Cat# 3591; RRID:AB_2097522
phospho-T37 4E-BP1	Cell Signaling Technology	Cat# 2855; RRID:AB_560835
phospho-T70 4E-BP1	Cell Signaling Technology	Cat# 9455; RRID:AB_330949
non-phospho-T46 4E-BP1	Cell Signaling Technology	Cat# 4923; RRID:AB_659944
phospho-S65 4E-BP1	Cell Signaling Technology	Cat# 9451; RRID:AB_330947
mTOR	Cell Signaling Technology	Cat# 2983; RRID:AB_2105622 #4517; RRID:AB_1904056
p70S6K1	Cell Signaling Technology	Cat# 9234; RRID:AB_2269803
Ero-1Lα	Cell Signaling Technology	Cat# 3264; RRID:AB_823684
PDI	Cell Signaling Technology	Cat# 3501; RRID:AB_2156433
DCP1B	Cell Signaling Technology	Cat# 13233; RRID:AB_2798157
DDX6	Cell Signaling Technology	Cat# 9407; RRID:AB_10556959
Tubulin	Cell Signaling Technology	Cat# 2128; RRID:AB_823664
CalR	Cell Signaling Technology	Cat# 2891; RRID:AB_2275208
PKCζ	Cell Signaling Technology	Cat# 9368; RRID:AB_10693777
PKC δ	Cell Signaling Technology	Cat# 2058; RRID:AB_10694655
BiP	Cell Signaling Technology	Cat# 3177; RRID:AB_2119845
LC3B	Cell Signaling Technology	Cat# 3868; RRID:AB_2137707
EEA1	Cell Signaling Technology	Cat# 3288; RRID:AB_2096811
AIF	Cell Signaling Technology	Cat# 5318; RRID:AB_10634755
Rab5	Cell Signaling Technology	Cat# 3547; RRID:AB_2300649
RCAS1	Cell Signaling Technology	Cat# 12290; RRID:AB_2736985
Calnexin (CNX)	Cell Signaling Technology	Cat# 2679; RRID:AB_2228381
PTBP1/2	Cell Signaling Technology	Cat# 8776 The product is discontinued
hnRNPK	Cell Signaling Technology	Cat# 9081; RRID:AB_11178946
FUBP/ KHSRP (E2E2U)	Cell Signaling Technology	Cat# 13398; RRID:AB_2798208
IRE1α	Cell Signaling Technology	Cat# 3294; RRID:AB_823545
rpS6	Cell Signaling Technology	Cat# 2217; RRID:AB_331355
rpL7a	Cell Signaling Technology	Cat# 2415; RRID:AB_2182059
SUN2	Abcam	Cat# ab124916; RRID:AB_10972497
Nesprin 1/Syne-1	Abcam	Cat# ab5250; RRID:AB_2200724
Exportin 5	Abcam	Cat# ab57491; RRID:AB_941499
SUN2	Sigma-Aldrich	Cat# MABT880
GRP78/HSPA5/BiP	Novus Biol	Cat# 06274; RRID:AB_1555284
eIF4E3	Proteintech	Cat# 17282-1-AP; RRID:AB_2262162
Bacterial and virus strains
ShRNA_eIF4E3 lentiviral particles	Santa Cruz Biotechnology	Cat# sc-78455-V
Mammalian vector pCMV6-AC-GFP (Human)	Origene	Cat# PS100010
Chemicals, peptides, and recombinant proteins
pp242	Tocris Bioscience	Cat# 4257/5
Kinesin inhibitor K858	Tocris Bioscience	Cat# 4960/1
Dynarrestin	Tocris Bioscience	Cat# 6526/5
Torin 1	Tocris Bioscience	Cat# 4247/10
PF4708671	Tocris Bioscience	Cat# 4032/5
Bio	Tocris Bioscience	Cat# 3194/10
Rotenone	Tocris Bioscience	Cat# 3616/50
MG132	Tocris Bioscience	Cat# 1748/5
Geldanamycin	Tocris Bioscience	Cat# 1368/1
T4 Polynucleotide Kinase	New England Biolabs	Cat# M0201S
T4 PK Reaction Buffer	New England Biolabs	Cat# B0201S
DMEM	Thermo Fisher Scientific	Cat# A4192101
RPMI 1640	Thermo Fisher Scientific	Cat# 61870143
FBS	Thermo Fisher Scientific	Cat# A4766
Penicillin/Streptomycin	Sigma	Cat# P4333
HEPES solution	Sigma	Cat# H0887
NaCl	Sigma	Cat# S5150
Glycerol	VWR	Cat# 24388-295
NP-40/IPEGAL	EMD Millipore	Cat# 492016
Triton X-100	Sigma	Cat# T8787
Tris-HCl pH 7.5 1M	Thermo Fisher Scientific	Cat# 15567027
RNAsin RNase inhibitor	Promega	Cat# N251B
Sodium deoxycholate	Ameresco	Cat# 0613
KCl	Thermo Fisher Scientific	Cat# AM9640G
MgCl2	Thermo Fisher Scientific	Cat# AM9530G
LiCl	Invitrogen	Cat# L7026
DTT	OmniPur	Cat# 3860
Cycloheximide	Ameresco	Cat# 94271-5G
RNase-free water	GE Healthcare	Cat# 0226
siRNA buffer	Dharmacon™	Cat# B-002000-UB-100
Protease Inhibitors	Roche	Cat# 11393100
qScript cDNA Super-Mix	QuantaBio	Cat# 10142-784
Formaldehyde	Sigma	Cat# F9037
MNase	Sigma-Aldrich	Cat# N3755
Guanidine thiocyanate	Sigma	Cat# 50983
Trizol	Ambion	Cat# 10296-010
GlycoBlue	ThermoFisher Scientific	Cat# AM9515
Polynucleotide kinase	BioLabs	Cat# M0201S
PBS	Gibco	Cat# 14200-075
Tween 20	Sigma-Aldrich	Cat# H06J02
Puromycin	GIBCO	Cat# J67236.8EQ
BSA	Sigma-Aldrich	Cat# A9647
Other
Compound	Target	Concentration
pp242	mTOR	500 nM
K858	Kinesin	1–10 µM
Dynarrestin	Dynein	5–25 µM
Torin 1	mTOR	250 nM
PF4708671	RSK	5 µM
Bio	GSK-3	1 µM
Rotenone	Mitochondrial electron transport	1 µM
MG132	26S proteasome	1 µM
Geldanamycin	Hsp90	1 µM
Critical commercial assays
RNeasy Mini Kit	QIAGEN	Cat# B-002000-UB-100
Stellaris RNA FISH	Biosearch technologies	Cat# VSMF-20680-5
QIAseq miRNA Library Prep Kit	QIAGEN	cat #331502
MTT Cell Growth Assay Kit	Sigma-Aldrich	Cat# CT02
Deposited data
mRNA and Ribo-seq data	NCBI GEO	GSE199535
The mass spectrometry proteomics data	ProteomeXchange Consortium	PRIDE: PXD031043
Experimental models: Cell lines
Colorectal carcinoma HCT-15	ATCC	CCL-225
Cervical adenocarcinoma HeLa	ATCC	CCL-2
Gastric adenocarcinoma AGS	ATCC	CRL-1739
Hepatocellular carcinoma Hep G2	ATCC	HB-8065
Experimental models: Organisms/strains
HCT-15, GFP- rpL7a expression	Origene	Cat# PS100010
HeLa, siRNA gene silencing, SUN2	Dharmacon™	Cat# 25777
HCT-15, Transduction of shRNA eIF4E3 lentiviral particles	Santa Cruz Biotechnology	Cat# sc-78455-V
Oligonucleotides
Human SUN2 siRNA	Dharmacon™	Cat# 25777
ShRNA_eIF4E3 lentiviral particles	Santa Cruz Biotechnology	Cat# sc-78455-V
Software and algorithms
Proteome Discoverer 2.1 software	Thermo Scientific	https://www.thermofisher.com/us/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-software/multi-omics-data-analysis/proteome-discoverer-software.html
Mascot 2.4	Matrix Science	https://www.matrixscience.com/
gpGrouper algorithm	Saltzman et al. (2018, MCP)85	PMID: 30093420
Gene Set Enrichment Analysis (GSEA)	Subramanian et al. (2005, PNAS)86	https://www.gsea-msigdb.org/gsea/index.jsp
FastQC	Andrews et al. (2010)87	https://www.bioinformatics.babraham.ac.uk/projects/fastqc/
Trimmomatic (v0.38)	Bolger et al., 201488	Bioinformatics 30, 2114-2120
Bowtie aligner (v1.3.0)	Langmead et al., 200989	Genome Biology 10, R25.
The STAR aligner (v2.7.6a)	Dobin et al., 201390	Bioinformatics 29, 15-21.
R package edgeR	Robinson et al., 201091	Bioinformatics 26, 139-140
RUV normalization	Risso et al., 201492	Nature biotechnology 32, 896-902
MSigDB	Liberzon et al., 201193	Bioinformatics 27, 1739-1740
MATLAB	Mathworks	https://www.mathworks.com/products/matlab.html
Image Studio Lite	LI-COR	https://www.licor.com/bio/image-studio-lite/
GraphPad Prism 9.4.1	GraphPad	https://www.graphpad.com/scientific-software/prism/
Adobe Illustrator CC 2022	Adobe	https://www.adobe.com/products/illustrator.html
NCBI GEO RNA-seq data	GEO accession GSE199535	https://www.ncbi.nlm.nih.gov/gds
ProteomeXchange Proteomic data	Dataset Identifier PXD 031043	http://proteomecentral.proteomexchange.org/cgi/GetDataset