Antibodies |
|
Polyclonal rabbit anti-AXT107 |
Covance Immunology Services |
PR191108B |
HRP-conjugated goat anti-rabbit IgG |
Cell Signaling Technologies |
Cat # 7074; RRID:AB_2099233
|
Integrin Alpha-V Beta-3 Antibody |
Abbiotec |
Cat# 251672; RRID:AB_10643501
|
Integrin Alpha-5 Beta-1 Antibody |
Novus Biological |
Cat # nbp2-29788 |
Anti-Integrin alpha5, C-terminus, intracellular antibody |
Millipore Sigma |
Cat# AB1928; RRID:AB_2128185
|
Anti-Integrin alphaV, C-terminus, Intracellular antibody |
Millipore Sigma |
Cat# AB1930; RRID:AB_390210
|
Alexa Fluor 488-conjugated donkey anti-rabbit IgG |
Thermo Fisher Scientific |
Cat# R37118; RRID:AB_2556546
|
Alexa Fluor 488-conjugated donkey anti-rat IgG |
Thermo Fisher Scientific |
Cat# A-21208; RRID:AB_2535794
|
DyLight 594-conjugated donkey anti-rabbit IgG |
Thermo Fisher Scientific |
Cat# SA5-10040; RRID:AB_2556620
|
Goat Anti-Mouse IgG Alexa Fluor 594 |
Abcam |
Cat# ab150116; RRID:AB_2650601
|
Normal Rabbit IgG antibody |
Millipore Sigma |
Cat# NI01-100UG; RRID:AB_490574
|
Normal rat IgG |
Millipore Sigma |
Cat# NI04 |
Alexa Fluor 488-conjugated goat anti-rabbit |
Thermo Fisher Scientific |
Cat# A-11034; RRID:AB_2576217
|
Alexa Fluor 594-conjugated goat anti-rabbit IgG |
Thermo Fisher Scientific |
Cat# A32740; RRID:AB_2762824
|
Alexa Fluor 594-conjugated goat anti-mouse IgG |
Thermo Fisher Scientific |
Cat# A32742; RRID:AB_2762825
|
|
Chemicals, peptides, and recombinant proteins |
|
Complete Protease Inhibitor Cocktail |
Sigma |
Cat# CO-RO |
Xylazine hydrochloride |
Covetrus |
SKU 061035 |
Ketamine hydrochloride |
Covetrus |
SKU 071069 |
Paraformaldehyde aqueous solution 32% |
Electron Microscopy Section |
Cat# 15714 |
Optimal Cutting Temperature (O.C.T.) |
Tissue Tek |
Cat# 4583 |
Tropicamide Ophthalmic solutions |
Alcon Labs, Inc. |
Cat# 61314–35502 |
AXT107 peptide |
Vivitide |
Lot#: LP02395-2 |
FAM-AXT107 |
GenScript |
Lot#: U086FGA110-1/PE3038 |
SP2048 |
Vivitide |
Lot#: A7118-4 |
SDS-loading buffer |
Cell Signaling Technologies |
Cat#: 56036 |
BSA |
Cell Signaling Technologies |
Cat#: 9998 |
Tween 20, Molecular Biology Grade |
Promega |
Cat#: H5151 |
Super-Signal West Pico PLUS |
Thermo Fisher Scientific |
Cat # PI34580 |
Normal goat serum |
Vector |
Cat # S-1000; RRID:AB_2336615
|
FITC-conjugated Griffonia Simplicifolia agglutinin lectin |
Vector |
Cat # FL-1101; RRID:AB_2336490
|
Lectin GS-II From Griffonia simplicifolia, Alexa Fluor™ 594 Conjugate |
Thermo Fisher Scientific |
Cat #L21416
|
Hoechst 33342 |
Thermo Fisher Scientific |
Cat #H3570 |
Aqueous Mounting AquaPoly solution |
Neobits |
Cat # 18606–5 |
Normal goat serum |
Thermo Fisher Scientific |
Cat# 31872, RRID:AB_2532166
|
Sulfo-SBED Biotin Label Transfer Reagent |
Thermo Fisher Scientific |
Cat# 33033 |
Streptavidin (Sepharose Bead Conjugate) |
Cell Signaling Technologies |
Cat#: 3419 |
|
Experimental models: Cell lines |
|
Human umbilical vein endothelial cells |
Lifeline Cell Technology |
FC-0044 |
VascuLife VEGF Endothelial Medium |
Lifeline Cell Technology |
LL-0003 |
|
Experimental models: Organisms/strains |
|
Mouse: C57BL/6J |
The Jackson Laboratories |
RRID:IMSRJAX:000664 |
Mouse: rho/VEGF transgenic mice |
The Campochiaro Laboratory |
B6-Tg (bRhdopsin-hVEGF-165) 6Pac |
|
Software and algorithms |
|
SPOT RT 4.6 software |
Diagnostic Instruments Inc. |
http://www.spotimaging.com/software/spot-advanced/; RRID:SCR_016613
|
ImageJ software |
NIH |
http://rsb.info.nih.gov/nih-image/index.html |
Infinity Capture v. 6.5.6 software |
Teledyne Lumenera |
https://infinity-software1.software.informer.com/6.5/ |
|
Other |
|
OcuLight GL Photocoagulator |
Iridex |
33003 EN |
Harvard Pump Microinjection System |
Harvard Appparatus |
Cat# 65–0001 |
Borosilicate Glass Capillaries |
World Precision Instruments |
Cat# TW100-4 |
iBright FL1000 imager |
Invitrogen |
N/A |
Zeiss Axioskop 2 Plus |
Carl Zeiss |
N/A |
Zeiss AxioObserver; LSM700 confocal module |
Carl Zeiss |
N/A |
Olympus IX71 |
Olympus Life Science |
N/A |
CL-1000 UV Crosslinker |
Analytik Jena |
N/A |