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. 2023 Jan 30;26(2):106078. doi: 10.1016/j.isci.2023.106078
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

Polyclonal rabbit anti-AXT107 Covance Immunology Services PR191108B
HRP-conjugated goat anti-rabbit IgG Cell Signaling Technologies Cat # 7074; RRID:AB_2099233
Integrin Alpha-V Beta-3 Antibody Abbiotec Cat# 251672; RRID:AB_10643501
Integrin Alpha-5 Beta-1 Antibody Novus Biological Cat # nbp2-29788
Anti-Integrin alpha5, C-terminus, intracellular antibody Millipore Sigma Cat# AB1928; RRID:AB_2128185
Anti-Integrin alphaV, C-terminus, Intracellular antibody Millipore Sigma Cat# AB1930; RRID:AB_390210
Alexa Fluor 488-conjugated donkey anti-rabbit IgG Thermo Fisher Scientific Cat# R37118; RRID:AB_2556546
Alexa Fluor 488-conjugated donkey anti-rat IgG Thermo Fisher Scientific Cat# A-21208; RRID:AB_2535794
DyLight 594-conjugated donkey anti-rabbit IgG Thermo Fisher Scientific Cat# SA5-10040; RRID:AB_2556620
Goat Anti-Mouse IgG Alexa Fluor 594 Abcam Cat# ab150116; RRID:AB_2650601
Normal Rabbit IgG antibody Millipore Sigma Cat# NI01-100UG; RRID:AB_490574
Normal rat IgG Millipore Sigma Cat# NI04
Alexa Fluor 488-conjugated goat anti-rabbit Thermo Fisher Scientific Cat# A-11034; RRID:AB_2576217
Alexa Fluor 594-conjugated goat anti-rabbit IgG Thermo Fisher Scientific Cat# A32740; RRID:AB_2762824
Alexa Fluor 594-conjugated goat anti-mouse IgG Thermo Fisher Scientific Cat# A32742; RRID:AB_2762825

Chemicals, peptides, and recombinant proteins

Complete Protease Inhibitor Cocktail Sigma Cat# CO-RO
Xylazine hydrochloride Covetrus SKU 061035
Ketamine hydrochloride Covetrus SKU 071069
Paraformaldehyde aqueous solution 32% Electron Microscopy Section Cat# 15714
Optimal Cutting Temperature (O.C.T.) Tissue Tek Cat# 4583
Tropicamide Ophthalmic solutions Alcon Labs, Inc. Cat# 61314–35502
AXT107 peptide Vivitide Lot#: LP02395-2
FAM-AXT107 GenScript Lot#: U086FGA110-1/PE3038
SP2048 Vivitide Lot#: A7118-4
SDS-loading buffer Cell Signaling Technologies Cat#: 56036
BSA Cell Signaling Technologies Cat#: 9998
Tween 20, Molecular Biology Grade Promega Cat#: H5151
Super-Signal West Pico PLUS Thermo Fisher Scientific Cat # PI34580
Normal goat serum Vector Cat # S-1000; RRID:AB_2336615
FITC-conjugated Griffonia Simplicifolia agglutinin lectin Vector Cat # FL-1101; RRID:AB_2336490
Lectin GS-II From Griffonia simplicifolia, Alexa Fluor™ 594 Conjugate Thermo Fisher Scientific Cat #L21416
Hoechst 33342 Thermo Fisher Scientific Cat #H3570
Aqueous Mounting AquaPoly solution Neobits Cat # 18606–5
Normal goat serum Thermo Fisher Scientific Cat# 31872, RRID:AB_2532166
Sulfo-SBED Biotin Label Transfer Reagent Thermo Fisher Scientific Cat# 33033
Streptavidin (Sepharose Bead Conjugate) Cell Signaling Technologies Cat#: 3419

Experimental models: Cell lines

Human umbilical vein endothelial cells Lifeline Cell Technology FC-0044
VascuLife VEGF Endothelial Medium Lifeline Cell Technology LL-0003

Experimental models: Organisms/strains

Mouse: C57BL/6J The Jackson Laboratories RRID:IMSRJAX:000664
Mouse: rho/VEGF transgenic mice The Campochiaro Laboratory B6-Tg (bRhdopsin-hVEGF-165) 6Pac

Software and algorithms

SPOT RT 4.6 software Diagnostic Instruments Inc. http://www.spotimaging.com/software/spot-advanced/; RRID:SCR_016613
ImageJ software NIH http://rsb.info.nih.gov/nih-image/index.html
Infinity Capture v. 6.5.6 software Teledyne Lumenera https://infinity-software1.software.informer.com/6.5/

Other

OcuLight GL Photocoagulator Iridex 33003 EN
Harvard Pump Microinjection System Harvard Appparatus Cat# 65–0001
Borosilicate Glass Capillaries World Precision Instruments Cat# TW100-4
iBright FL1000 imager Invitrogen N/A
Zeiss Axioskop 2 Plus Carl Zeiss N/A
Zeiss AxioObserver; LSM700 confocal module Carl Zeiss N/A
Olympus IX71 Olympus Life Science N/A
CL-1000 UV Crosslinker Analytik Jena N/A