Activation of innate antiviral response mediated by phospho-PKR, phospho-c-Jun, and phospho-c-Fos in infected HeLa cells following oleuropein treatment. HeLa cells were infected at 10 MOI with HSV-1 and were untreated or treated with different concentrations of oleuropein (400 µg/mL, 300 µg/mL, and 100 µg/mL). (A–C) Phospho-PKR, phospho-c-Jun, and phospho-c-Fos protein expression was evaluated via Western blotting. Quantitation of the respective phospho-PKR, phospho-c-Fos and phospho-c-Jun protein bands is shown in (B,D). Data represent the means of 3 independent experiments ± SD. Statistical evaluation via one-way ANOVA: * p < 0.1, **** p < 0.001.