Figure 3.

LMW-AP-FBG induces apoptosis in CT-26 cells. CT-26 cells were treated with indicated concentrations of LMW-AP-FBG for 24 h. Apoptosis was measured after staining cells with Annexin V and 7-AAD. (a) Representative annexin V dot plots are shown. (b) Percentages of cells in early and (c) late apoptosis in CT-26 cells are marked on a graph. (d) Representative dot plots of caspase activity measurement with a Muse™ Multi-caspase Kit. (e) Percentages of caspase activities were marked on a graph. Results are presented as mean ± SEM of three independent experiments for triplicate. * p < 0.05, *** p < 0.001, compared with untreated control cells (0 mg/mL).