Expression profiles of factors related to hair growth during the depilation-induced hair cycle. The dorsal skins of C57BL/6N 7-week-old wild-type male mice were plucked and harvested at 0, 6, 9, 16, or 18 days. (a) H&E staining for dorsal skins. (b) IHC staining for Ptgds, p-smad1/5/9, Cxxc5, and β-catenin. (c) Immunoblotting assay of whole skin for Ptgds, p-smad1/5/9, Cxxc5, β-catenin, and total Erk. (d) qRT-PCR of whole skin for Ptgds, Cxxc5, Hes1, and Jag1 (n = 5). Scale bars = 100 µm. Dashed lines indicate hair follicles. Values are expressed as the means ± SEM. Student’s t-test: * p < 0.05, ** p < 0.005, *** p < 0.0005, N.S: not significant.