Table 2.
Correlation of log10IC50 values for odoroside H and neritaloside with ABC-transporter mediated mechanisms of multidrug resistance (P-glycoprotein/ABCB1, ABCB5, ABCC1, and ABCG2) and other mechanisms of anticancer drug resistance (EGFR, RAS, TP53, WT1, HSP90, GSTπ, and the proliferation rate of the tumor cell lines).
| Odoroside H | Neritaloside | Control Drug | ||
|---|---|---|---|---|
| (log10IC50, M) | (log10IC50, M) | (log10IC50, M) | ||
| ABCB1 Expression | Epirubicin | |||
| 7q21 (Chromosomal | r-value | −0.039 | 0.004 | * 0.447 |
| Locus of ABCB1 Gene) | p-value | 0.390 | 0.489 | * 3.55 × 10−4 |
| ABCB1 Expression | r-value | −0.051 | −0.022 | * 0.533 |
| (Microarray) | p-value | 0.350 | 0.436 | * 6.82 × 10−6 |
| ABCB1 Expression | r-value | −0.036 | −0.104 | * 0.410 |
| (RT-PCR) | p-value | 0.401 | 0.233 | * 1.54 × 10−3 |
| Rhodamine 123 | r-value | −0.023 | 0.022 | * 0.526 |
| Accumulation | p-value | 0.433 | 0.435 | * 1.12 × 10−5 |
| ABCB5 Expression | Maytansine | |||
| ABCB5 Expression | r-value | 0.107 | 0.140 | * 0.454 |
| (Microarray) | p-value | 0.209 | 0.143 | * 6.67 × 10−4 |
| ABCB5 Expression | r-value | 0.140 | 0.227 | * 0.402 |
| (RT-PCR) | p-value | 0.143 | * 0.040 | * 0.0026 |
| ABCC1 Expression | Vinblastine | |||
| DNA Gene | r-value | 0.008 | 0.010 | * 0.429 |
| Copy Number | p-value | 0.477 | 0.233 | * 0.001 |
| ABCC1 Expression | r-value | −0.141 | −0.254 | * 0.398 |
| (Microarray) | p-value | 0.145 | * 0.027 | * 0.003 |
| ABCC1 Expression | r-value | −0.151 | −0.265 | 0.299 |
| (RT-PCR) | p-value | 0.156 | 0.0357 | * 0.036 |
| ABCG2 Expression | Pancratistatin | |||
| ABCG2 Expression | r-value | −0.099 | −0.150 | * 0.329 |
| (Microarray) | p-value | 0.230 | 0.131 | * 0.006 |
| ABCG2 Expression | r-value | −0.049 | −0.058 | * 0.346 |
| (Western Blot) | p-value | 0.356 | 0.332 | * 0.004 |
| EGFR Expression | Erlotinib | |||
| EGFR Gene | r-value | 0.013 | −0.194 | −0.245 |
| Copy Number | p-value | 0.459 | 0.069 | * 0.029 |
| EGFR Expression | r-value | 0.090 | −0.275 | * −0.458 |
| (Microarray) | p-value | 0.248 | * 0.017 | * 1.15 × 10−4 |
| EGFR Expression | r-value | 0.143 | −0.111 | * −0.379 |
| (PCR Slot Blot) | p-value | 0.143 | 0.203 | * 0.002 |
| EGFR Expression | r-value | −0.052 | −0.293 | * −0.376 |
| (Protein Array) | p-value | 0.349 | * 0.012 | * 0.001 |
| N-/K-/H-RAS Mutations | Melphalan | |||
| TP53 Mutation | r-value | 0.131 | 0.182 | * 0.367 |
| (cDNA Sequencing) | p-value | 0.161 | 0.084 | * 0002 |
| TP53 Mutation | 5-Fluorouracil | |||
| TP53 Mutation | r-value | −0.222 | −0.218 | * −0.502 |
| (cDNA Sequencing) | p-value | 0.047 | 0.050 | * 3.50 × 10−5 |
| TP53 Function | r-value | −0.119 | 0.115 | * −0.436 |
| (Yeast Functional Assay) | p-value | 0.195 | 0.204 | * 5.49 × 10−4 |
| WT1 Expression | Ifosfamide | |||
| WT1 Expression | r-value | −0.019 | 0.010 | * −0.316 |
| (Microarray) | p-value | 0.442 | 0.469 | * 0.007 |
| GSTP1 Expression | Etoposide | |||
| GSTP1 Expression | r-value | −0.124 | −0.071 | 0.399 |
| (Microarray) | p-value | −0.0173 | 0.296 | * 9.58 × 10−4 |
| GST Expression | r-value | 0.008 | −0.064 | 0.509 |
| (Northern Blot) | p-value | 0.474 | 0.315 | * 2.24 × 10−5 |
| HSP90 Expression | Geldanamycin | |||
| HSP90 Expression | r-value | −0.011 | 0.466 | * −0.392 |
| (Microarray) | p-value | 0.076 | 0.283 | * 0.001 |
| Proliferation | 5-Fluorouracil | |||
| Cell Doubling | r-value | 0.079 | −0.008 | * 0.627 |
| p-value | 0.279 | 0.477 | * 7–14 × 10−6 |
* r > 0.3 or <−0.3 and p < 0.05.