Skip to main content
. 2023 Feb 14;14(2):189. doi: 10.3390/insects14020189

Figure 5.

Figure 5

Effects of RNAi-mediated knockdown of AeELO2 and AeELO9 on egg production and hatchability. (A) RNAi-mediated knockdown of AeELO2. (B) RNAi-mediated knockdown of AeELO9. (C) Changes of oviposition in adult mosquitoes after injection with dsRNAs targeting AeELO2 and AeELO9. The statistical unit is a single mosquito. (D) Effect of RNAi-mediated knockdown of AeELO2 and AeELO9 on egg hatching rates. (E) RNAi-mediated knockdown of AeELO2 and AeELO9 reduced egg length. Abbreviations: DEPC, blank control, adult mosquitoes treated with diethyl pyrocarbonate water; GUS, adult mosquitoes treated with gus-dsRNA; ELO2, adult mosquitoes treated with AeELO2-dsRNA; ELO9, adult mosquitoes treated with AeELO9-dsRNA; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns, not significant. *, **, etc., represent a significant difference compared with the blank control (adult mosquitoes treated with DEPC water). Red represents AeELO2 and green represents AeELO9; The black dot, gray box, red triangle, and green inverted triangle represent the number of eggs laid by DEPC water, gus-dsRNA, AeELO2, and AeELO9, respectively.