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. 2023 Feb 14;14(2):189. doi: 10.3390/insects14020189

Figure 6.

Figure 6

Effects of RNAi-mediated knockdown of AeELO2 and AeELO9 on adult mosquito cuticles and egg development. (A) Alterations in cuticular elasticity in adult mosquitoes after AeELO2 knockdown. Here, 1, 2 show the AeELO2 knockdown group; 3 and 4 show the gus-dsRNA-treated and DEPC water-treated control groups, respectively. (B) Same as panel a, and 1 and 3 represent the gus-dsRNA-treated and DEPC-water treated control groups, respectively; 2 represents the AeELO2 knockdown group. (C) The phenotype of the hatched eggs, where 1 and 2 represent the gus-dsRNA-treated and DEPC water-treated control groups, respectively; 3 and 4 showed the AeELO2 knockdown group. (D,E) Alterations in cuticular osmotic pressure in adult mosquitoes after AeELO9 knockdown. Here, 1 and 3 represent the gus-dsRNA treated and DEPC water-treated control groups, respectively, in panels d and e; 2 AeELO9-dsRNA-treated mosquitoes. (F) Status of unhatched eggs in the AeELO9 knockdown group. Here, 1 and 2 show the gus-dsRNA treated and DEPC water-treated control groups, respectively; 3 represents the AeELO9 knockdown group. Abbreviations: DEPC, diethyl pyrocarbonate. (A,B,D,E) Each treatment group contained 30 adults, and the experiment was repeated three times. (C,F) Each treatment group dissected 60 unhatched eggs, and the experiment was repeated three times.