Developmental Biology Correction for “The multifaceted role of GCM1 during trophoblast differentiation in the human placenta,” by Mariyan J. Jeyarajah, Gargi Jaju Bhattad, Rachel D. Kelly, Kelly J. Baines, Adam Jaremek, Fei-Hung P. Yang, Hiroaki Okae, Takahiro Arima, Vanessa Dumeaux, and Stephen J. Renaud, which published November 28, 2022; 10.1073/pnas.2203071119 (Proc. Natl. Acad. Sci. U.S.A. 119, e2203071119).
The authors note that Fig. 2 appeared incorrectly. Specifically, one of the phase images in Fig. 2C and the loading control in the Western blot shown in Fig. 2B were inadvertently swapped. The corrected figure and its legend appear below. The online version has been corrected.
Fig. 2.
GCM1 is required for EVT development. (A) Quantitative RT-PCR and (B) Western blot analysis showing GCM1 expression in control (CTRL) and two distinct GCM1 knockdown (KD1 and KD2) TS cells differentiated into EVTs. ACTB was used as a loading control for Western blotting. (C) Phase-contrast images depicting CTRL, GCM1 KD1, and GCM1 KD2 cells. (D) Quantitative RT-PCR analysis showing expression of one CT marker (TEAD4) and three EVT markers (HLA-G, ITGA1, and MMP2) in CTRL, GCM1 KD1, and GCM1 KD2 TS cells differentiated into EVTs. (E) Quantification of percent EdU-positive nuclei in CTRL and GCM1 KD1 TS cells in CT stem state or differentiated into EVTs for 6 d. (F) Relative number of CTRL, GCM1 KD1, and GCM1 KD2 TS cells that invaded through Matrigel following differentiation into EVTs. Representative images of invaded cells are found to the right of the graph. Membrane pores are the black circles; cells are stained purple. (G) Principal component analysis showing the relationship between replicates of CTRL and GCM1 KD1 cells cultured in CT or EVT media, using all expressed genes as input. Please note that CTRL and KD1 cells cluster close together in CT media but diverge when cultured in EVT media. (H) Volcano plot showing the number of unique transcripts identified in RNAseq analysis. Transcripts up-regulated in GCM1 KD1 EVTs relative to CTRL EVTs are shown in red (fold change ≥ 2, P < 0.01), and down-regulated transcripts are shown in blue. The X-axis represents the magnitude of fold change, and the Y-axis shows the P-value. Values significantly different between CTRL CT and CTRL EVT are indicated with an asterisk (*P < 0.05), and values significantly different between CTRL EVT and GCM1 KD EVT are indicated with a number sign (#P < 0.05); ns, not statistically significant. N = 6 for A and D, all other panels are N = 3. (Scale bar, 100 µm.)