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Qualitative ROS generation in SAEC after 24 h and 7 days of ENDS aerosol exposure at two different device-aging stages. At 24 h, Juul Virginia Tobacco exposures elicited significant levels of ROS (C,D) in comparison to the negative and blank control (A,B) at both puff fractions 1–50 and 101–150. After 7 days of repeated JUUL exposure, each puff fraction elevated ROS levels (G,H) in SAEC relative to the negative and blank control (E,F).
