Quantitative data analysis of ROS generation in SAEC after 24 h (A) and 7 days (B) exposure at two puff fractions (1–50) and (101–150). After 24 h of exposure, there was a significant increase (***, p < 0.001) in ROS generation at the later puff fraction (101–150) compared to negative control (A). There was significantly higher (**, p < 0.01) relative fluorescence intensity detected in the 101–150 puffs than the 1–50 puffs at 24-h (A). Similarly, after 7 days of repeated exposure, the later puff fraction (101–150) elicited significantly higher (**, p < 0.01) ROS than the 1–50 puff fraction (B).